Overview

A valuable collection of new and unique techniques for the construction and use of peptide libraries. These powerful methods include protocols for the chemical synthesis of peptide libraries, for constructing peptide libraries that are displayed on the surface of filamentous phage or bacteria, and for the rapid screening of these libraries for molecules with biospecific properties. Additional methods permit identifying specific enzyme substrates, investigating the recognition spectra of various binding proteins, epitope mapping, and identifying mimotopes. State-of-the-art techniques, combined with clear step-by-step instructions, make this book an essential tool in the selection of peptides suitable for drug development.

Full Product Details

Author:   Shmuel Cabilly
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1998 ed.
Volume:   87
Dimensions:   Width: 15.50cm , Height: 2.60cm , Length: 23.50cm
Weight:   0.725kg
ISBN:  

9780896033924

ISBN 10:   0896033929
Pages:   313
Publication Date:   23 December 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Synthesis of a One-Bead One-Compound Combinatorial Peptide Library.- Enzyme-Linked Calorimetric Screening of a One-Bead One-Compound Combinatorial Library.- Synthesis and Screening of Positional Scanning Combinatorial Libraries.- Synthesis and Screening of Peptide Libraries on Continuous Cellulose Membrane Supports.- Peralkylation.- to Combinatorial Solid-Phase Assays for Enzyme Activity and Inhibition.- Preparation of Biocompatible Resins for Library Syntheses.- Intramolecular Fluorescence-Quenched Substrate Libraries.- The Solid-Phase Enzyme Inhibitor Library Assay.- Determination of Peptide Substrate Motifs for Protein Kinases Using a “One-Bead One-Compound” Combinatorial Library Approach.- The Use of Peptide Library for the Determination of Kinase Peptide Substrates.- Analysis of Protein Kinase Substrate Specificity by the Use of Peptide Libraries on Cellulose Paper (SPOT-Method).- Generation of Multiuse Peptide Libraries for Functional Screenings.- Functional Screening of Multiuse Peptide Libraries Using Melanophore Bioassay.- The Basic Structure of Filamentous Phage and Its Use in the Display of Combinatorial Peptide Libraries.- Construction and Use of a 20-mer Phage Display Epitope Library.- Construction of Disulfide-Constrained Random Peptide Libraries Displayed on Phage Coat Protein VIII.- Conformational Mimicry Through Random Constraints Plus Affinity Selection.- The Use of Combinatorial Libraries to Identify Ligands That Interact with Surface Receptors in Living Cells.- Screening Phage Display Peptide Libraries on Nitrocellulose Membranes.- Identification of Disease-Specific Epitopes.- Identification of Peptide Ligands for the Antigen Binding Receptor Expressed on Human B-Cell Lymphomas.- Major Histocompatibility Complex Allele-Specific Peptide Librariesand Identification of T-Cell Mimotopes.- Phage Display of Peptide Libraries on Protein Scaffolds.- Displaying Libraries of Conformationally Constrained Peptides on the Surface of Escherichia coli as Flagellin Fusions.- Combinatorial Peptide Library as an Immunogen.

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