Overview

In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. The book integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. Experimental approaches are thoroughly detailed and explained through a step-by-step instructional format that ensures successful results.

Full Product Details

Author:   Antonia Vlahou
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2008
Volume:   428
Dimensions:   Width: 15.50cm , Height: 2.10cm , Length: 23.50cm
Weight:   0.640kg
ISBN:  

9781617377990

ISBN 10:   1617377996
Pages:   408
Publication Date:   19 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Preface.- Acknowledgments.- Contributors.- 1. Overview and Introduction to Clinical Proteomics Young-Ki Paik, Hoguen Kim, Eun-Young Lee, Min-Seok Kwon, and Sang Yun Cho.- I: Specimen Collection for Clinical Proteomics.- 2. Specimen Collection and Handling: Standardization of Blood Sample CollectionHarald Tammen.- 3. Tissue Sample Collection for Proteomics Analysis Jose I. Diaz, Lisa H. Cazares, and O. John Semmes.- II: Clinical Proteomics by 2DE and Direct MALDI/SELDI MS Profiling.- 4. Protein Profiling of Human Plasma Samples by Two-Dimensional Electrophoresis Sang Yun Cho, Eun-Young Lee, Hye-Young Kim, Min-Jung Kang, Hyoung-Joo Lee, Hoguen Kim, and Young-Ki Paik.- 5. Analysis of Laser Capture Microdissected Cells by 2-Dimensional Gel Electrophoresis Daohai Zhang and Evelyn Siew-Chuan Koay.- 6. Optimizing the Difference Gel Electrophoresis (DIGE) Technology David B. Friedman and Kathryn S. Lilley.- 7. MALDI/SELDI Protein Profiling of Serum for the Identification of Cancer Biomarkers Lisa H. Cazares, Jose I. Diaz, Rick R. Drake, and O. John Semmes.- 8. Urine Sample Preparation and Protein Profiling by Two-Dimensional Electrophoresis and Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectroscopy Panagiotis G. Zerefos and Antonia Vlahou.- 9. Combining Laser Capture Microdissection and Proteomics Techniques Dana Mustafa, Johan M. Kros, and Theo Luider.- III: Clinical Proteomics by LC-MS Approaches.- 10. Comparison of Protein Expression by Isotope-Coded Affinity Tag Labeling Zhen Xiao and Timothy D. Veenstra.- 11. Analysis of Microdissected Cells by Two-Dimensional LC-MS Approaches Chen Li, Yi-Hong, Ye-Xiong Tan, Jian-Hua Ai, Hu Zhou, Su-Jun Li, Lei Zhang, Qi-Chang Xia, Jia-Rui Wu, Hong-Yang Wang, and Rong Zeng.- 12. Label-Free LC-MS Method for the Identification of Biomarkers Richard E. Higgs, Michael D. Knierman, Valentina Gelfanova, Jon P. Butler, and John E. Hale.- 13. Analysis of the Extracellular Matrix and Secreted Vesicle Proteomes by Mass Spectrometry Zhen Xiao, Thomas P. Conrads, George R. Beck, Jr., and Timothy D. Veenstra.- IV: Clinical Proteomics and Antibody Arrays.- 14. Miniaturized Parallelized Sandwich Immunoassays Hsin-Yun Hsu, Silke Wittemann, and Thomas O. Joos.- 15. Dissecting Cancer Serum Protein Profiles Using Antibody Arrays Marta Sanchez-Carbayo.- V: Statistics and Bioinformatics in Clinical Proteomics Data Analysis.- 16. 2D-PAGE Maps Analysis Emilio Marengo, Elisa Robotti, and Marco Bobba.- 17. Finding the Significant Markers: Statistical Analysis of Proteomic DataSebastien Christian Carpentier, Bart Panis, Rony Swennen, and Jeroen Lammertyn.- 18. Web-Based Tools for Protein Classification Costas D. Paliakasis, Ioannis Michalopoulos, and Sophia Kossida.- 19. Open-Source Platform for the Analysis of Liquid Chromatography-Mass Spectrometry (LC-MS) Data Matthew Fitzgibbon, Wendy Law, Damon May, Andrea Detter, and Martin McIntosh.- 20. Pattern Recognition Approaches for Classifying Proteomic Mass Spectra of Biofluids Ray L. Somorjai.

Reviews

From the reviews: Clinical proteomics is a fledgling field that is struggling to define its standards and whose underlying technologies continue to evolve at a very rapid pace. ... This multiauthored book provides state-of-the-art information and practical protocols on these topics and many other related areas. ... provides a wealth of background information and underlying principles. ... Overall, this is an excellent book whose natural readership includes both newcomers to the field and seasoned biomarker hunters who wish to update their knowledge or explore new techniques. (Marco Crescenzi, Annali dell'Istituto Superiore di Sanita, Vol. 44 (3), November, 2008)

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