Overview

"The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (for example, histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information for many aspects of cellular genetics such as DNA replication, The book is structured in a methodical fashion - the introductory chapters are centred around analysis of chromatin with chapters on the mapping of protein: DNA interactions ""in vivo"" using ligation-mediated PCR and the mapping of chromatin-associated proteins by formaldehyde cross-linking. The next chapters concentrate on the study of whole chromosome structure, including: fission yeast chromosome analysis using FISH and CHIP, isolation of vertebrate metaphase chromosomes and their analysis by FISH, the study of vertebrate chromosome progression through mitosis, and the analysis of mammalian interphase chromosomes by immunofluorescence and FISH. There then follow chapters on FISH in whole-mount tissues and the analysis of the sub-structure of mammalian nuclei ""in vitro"". protein, DNA interactions and genetic manipulation This bo"

Full Product Details

Author:   Wendy A. Bickmore
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   No. 200
Dimensions:   Width: 15.00cm , Height: 1.80cm , Length: 24.00cm
Weight:   0.421kg
ISBN:  

9780199636990

ISBN 10:   0199636990
Pages:   209
Publication Date:   01 July 1999
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

"1: Mapping protein: DNA interactions ""in vivo"" using ligation-mediated Polymerase Chain Reaction; 2: Mapping DNA target sites of chromatin-associated proteins by formaldehyde cross-linking in Drosophila embryos; 3: Fission yeast chromosome analysis: Fluorescence ""in situ"" hybridization (FISH) and chromatin immunoprecipitation; 4: Isolation of vertebrate metaphase chromosomes and their analysis by FISH; 5: Studying progression of vertebrate chromosomes through mitosis by immunoflourescence and FISH; 6: Analysis of mammalian interphase chromosomes by FISH and immunofluorescence; 7: Fluorescence ""in situ"" hybridization in whole-mount tissues; 8: Analyzing the sub-structure of mammalian nuclei, ""in vitro""; 9: Chromosome assembly ""in vitro"" using Xenopus egg extracts; 10: Chromosome fragmentation in vertebrate cell lines."

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