Overview

This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Chromosome Analysis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

Full Product Details

Author:   Eisuke Gotoh
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2519
Weight:   0.591kg
ISBN:  

9781071624326

ISBN 10:   1071624326
Pages:   193
Publication Date:   07 September 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

"1. A Basic and Simple Chromosome Preparation Protocol Eisuke Gotoh   2. Metaphase chromosome preparation and classification of chromosomal aberrations Takamitsu A Kato   3. Mitotic Index Analysis Takamitsu A Kato and Jeremy S. Haskins   4. Preparation of Mitotic Cells for Fluorescence Microscopy Yuji Tanno   5. Chemical induced Premature Chromosome Condensation protocol Eisuke Gotoh   6. Apoptosis Detection Assays Ilham Alshiraihi  and Takamitsu A Kato   7. Alkaline Comet Assay To Detect DNA Damage Kade D. Walsh and Takamitsu A Kato   8. Analysis for Sister Chromatid Exchange Takamitsu A Kato   9. Cytokinesis Blocked Micronuclei Aberration Analysis Takamitsu A Kato   10. DNA Damage Foci on Metaphase Chromosomes Takamitsu A Kato   11. FISH with Whole Chromosome Painting Probes Takamitsu A Kato   12. Telomere aberration detection by PNA-FISH probe Takamitsu A Kato   13. Nontraditional Method for Telomere Staining by PNA Probes Takamitsu A Kato   14. Visualizing Active Replication Regions in S-phase Chromosomes Eisuke Gotoh   15. Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer Atsushi Okabe and Atsushi Kaneda   16. CUT&Tag using ""stress-free"" con A-conjugated Dynabeads® Yasuhiro Fujiwara and Yuki Okada   17. Live-cell Synthetic Histone Acetylation by Chemical Catalyst Shigehiro A. Kawashima and Motomu Kanai   18. Histone Modification Analysis of Low-Mappability Regions Naoko Yoshizawa-Sugata and Hisao Masai1. A Basic and Simple Chromosome Preparation Protocol Eisuke Gotoh   2. Metaphase chromosome preparation and classification of chromosomal aberrations Takamitsu A Kato   3. Mitotic Index Analysis Takamitsu A Kato and Jeremy S. Haskins   4. Preparation of Mitotic Cells for Fluorescence Microscopy Yuji Tanno   5. Chemical induced Premature Chromosome Condensation protocol Eisuke Gotoh   6. Apoptosis Detection Assays Ilham Alshiraihi  and Takamitsu A Kato   7. Alkaline Comet Assay To Detect DNA Damage Kade D. Walsh and Takamitsu A Kato   8. Analysis for Sister Chromatid Exchange Takamitsu A Kato   9. Cytokinesis Blocked Micronuclei Aberration Analysis Takamitsu A Kato   10. DNA Damage Foci on Metaphase Chromosomes Takamitsu A Kato   11. FISH with Whole Chromosome Painting Probes Takamitsu A Kato   12. Telomere aberration detection by PNA-FISH probe Takamitsu A Kato   13. Nontraditional Method for Telomere Staining by PNA Probes Takamitsu A Kato   14. Visualizing Active Replication Regions in S-phase Chromosomes Eisuke Gotoh   15. Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer Atsushi Okabe and Atsushi Kaneda   16. CUT&Tag using ""stress-free"" con A-conjugated Dynabeads® Yasuhiro Fujiwara and Yuki Okada   17. Live-cell Synthetic Histone Acetylation by Chemical Catalyst Shigehiro A. Kawashima and Motomu Kanai   18. Histone Modification Analysis of Low-Mappability Regions Naoko Yoshizawa-Sugata and Hisao Masai"

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