Overview

Chromatin comprises the complex of nucleic acids and proteins that make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin should find this book useful. It should also be of interest to experienced researchers.

Full Product Details

Author:   Hannah Gould (Professor, Development Biology Research Centre, King's College, London)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   189
Dimensions:   Width: 15.70cm , Height: 1.40cm , Length: 23.40cm
Weight:   0.438kg
ISBN:  

9780199635986

ISBN 10:   0199635986
Pages:   248
Publication Date:   30 April 1998
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

1: Isolation and fractionation of chromatin and linker histones 2: Extraction and separation of core histones and non-histone chromosomal proteins 3: Mapping chromatin structure in Drosophila 4: In vivo footprint and chromatin analysis by LMPCR 5: Analysis of nuclear scaffold attachment regions 6: Electron microscopy of chromatin 7: Counting nucleosome cores on circular DNA using topoisomerase I 8: In vitro reconstitution of chromatin and analysis of nucleosome positioning 9: Transcriptional and structural analysis of chromatin assembled in vivo 10: An assembly of chromatin and nuclear structures in Xenopus egg extracts Appendix: List of Suppliers Index

Reviews

<br> The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology<br>

The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methodscapable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately thenatural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and establishedinvestigators will make use of this volume. --The Quarterly Review of Biology

<br> The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology<p><br>

The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology<br>

The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume.--The Quarterly Review of Biology

The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume.--The Quarterly Review of Biology

The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume. --The Quarterly Review of Biology

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