Overview

The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function.  In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.

Full Product Details

Author:   Gerard Drewes ,  Marcus Bantscheff
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2012
Volume:   803
Dimensions:   Width: 17.80cm , Height: 1.80cm , Length: 25.40cm
Weight:   0.849kg
ISBN:  

9781493958009

ISBN 10:   1493958003
Pages:   313
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

 Mass Spectrometry-Based Chemoproteomic Approaches.-Chemical Proteomics in Drug Discovery.-Compound Immobilization and Drug-Affinity Chromatography.-Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates.- A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine.-Probing Proteomes with Benzophenone Photoprobes.-Biotinylated Probes for the Analysis of Protein Modification by Electrophiles.-Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture Compound Mass Spectrometry (CCMS).-Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC.-Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags.-Affinity-Based Profiling of Dehydrogenase Subproteomes.- Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics.- Fluorescence-Based Proteasome Activity Profiling.- Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions.- Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry.- Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies.-Ligand Discovery Using Small-Molecule Microarrays.-Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of Kinetic Solubility.-A Database for Chemical Proteomics - ChEBI.-Working with Small Molecules: Rules-of-Thumb of “Drug Likeness”                        

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