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OverviewCD83 is one of the best-known surface markers for mature dendritic cells (DC). As CD83 is specifically upregulated upon DC maturation, a highly cell type- and maturation status-specific gene regulation is hypothesized. To elucidate the regulation mechanisms of CD83 expression in human DC, ChIP-chip-microarray-, biocomputational-, reporter, CpG-methylation- and EMSA -analyses were performed. By these studies, a tripartite transcriptional activation complex composed of an upstream regulatory element, a minimal promoter and an enhancer was identified. Their specific arrangement and cooperation in trans, mediated by a complex framework of IRF- and NFkB-binding sites, has not been reported for any other gene so far. The characterization of the CD83 promoter complex not only allows a detailed look into the elaborate mechanisms of the immune response in general and the biology of DC in particular, but also exemplarily unfolds the cooperation of various promoter elements in a highly cell type- and status-specific immune cell related promoter. Thus, for the first time, a human mature DC-specific promoter is described that might become an important tool for transcriptional targeting of DC. Full Product DetailsAuthor: Marcello SteinPublisher: Sudwestdeutscher Verlag Fur Hochschulschriften AG Imprint: Sudwestdeutscher Verlag Fur Hochschulschriften AG Dimensions: Width: 15.20cm , Height: 1.40cm , Length: 22.90cm Weight: 0.358kg ISBN: 9783838136684ISBN 10: 3838136683 Pages: 240 Publication Date: 08 March 2013 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In stock We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |