Cereal Genomics: Methods and Protocols

Author:   Luis M. Vaschetto
Publisher:   Humana Press Inc.
Edition:   1st ed. 2020
Volume:   2072
ISBN:  

9781493998678


Pages:   247
Publication Date:   20 September 2020
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Cereal Genomics: Methods and Protocols


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Overview

The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Luis M. Vaschetto
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2020
Volume:   2072
Weight:   0.500kg
ISBN:  

9781493998678


ISBN 10:   1493998676
Pages:   247
Publication Date:   20 September 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

A Low-Cost High-throughput Method for Plant Genomic DNA Isolation.- Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement.- Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping.- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops.- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing.- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR.- DNA Methylation and Transcriptomic Next Generation Technologies in Cereal Genomics.- Genome-wide Identification of Regulatory DNA Elements in Crop Plants.- Genome-wide Profiling of Histone Modifications with ChIP-seq.- Whole-genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes.- Genome-wide Identification of Allele-Specific Gene Expression in a Parent‑of‑Origin Specific Manner.- Analysis of Epigenetic Modifications during Vegetative and Reproductive Development in Cereals using Chromatin Immunoprecipitation (ChIP).- Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis.- Emerging Genome Engineering Tools in Crop Research and Breeding.- CRISPR/Cas9-mediated Targeted Mutagenesis in Wheat Doubled Haploids.- Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne.- Agrobacterium Transformation in the Rice Genome.- In vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays.

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