Overview

There are three main themes running through this volume. First, basic methods for measurement of cell proliferation are introduced and explained with reference to various systems, primarily in vitro, but in vivo procedures are also illustrated. The second theme is growth signalling, and is exemplified by methods for the analysis of transduction pathways for growth, beginning at the cell membrane and leading to the cell nucleus. The last theme presented here is growth cessation, illustrated by several systems for induction of cell differentiation, and of cell senescence. The emphasis throughout the book is on human cell systems, making it particularly relevant to scientists interested in human disease, especially cancer. Importantly, well proved methods for studying cell growth are supplemented by some novel approaches, e.g., studies of cell cycle checkpoints, cell spheroids, and nuclear architecture. Only two chapters have been retained, in an updated form from Cell Growth and Apoptosis, the predecessor volume. The book is written by a team of scientists highly experienced in procedures they describe, and offer details and hints found valuable in their own laboratories; thus, variants of the same general methods can be found in different chapters. These should be helpful to beginning as well as experienced investigators, and are designed to stimulate new approaches to old and new questions.

Full Product Details

Author:   George Studzinski (Professor of Pathology and Laboratory Medicine, New Jersey Medical School)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   215
Dimensions:   Width: 15.50cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.543kg
ISBN:  

9780199637683

ISBN 10:   0199637687
Pages:   332
Publication Date:   25 November 1999
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Preface; Chapter 1 Wieder: Selection of Methods for Measuring Proliferation; Chapter 2 Skehan: Cell Growth and Cytotoxicity Assays; Chapter 3 Durand: Cell Growth and Kinetics in Multicell Spheroids; Chapter 4 O'Connor & Jackman: Assessment of Checkpoint Controls of Cell Growth; Chapter 5 Bishayee: Assessment of the Role of Growth Factors and their Receptors in Cell Proliferation; Chapter 6 Alessandrini: Analysis of Growth-Signaling Protein Kinase Cascades; Chapter 7 Juan et al: Cytometric Analysis of the pRB Pathway; Chapter 8 Cosenza et al: Methods for Oncogene Detection; Chapter 9 Stein et al: Isolation and Visualization of the Principal Components of Nuclear Architecture; Chapter 10 Wolf & Flanagan: Antisense Oligonucleotides - tools for elucidating gene function; Chapter 11 Moore & Studzinski: Human Leukemia Cells as a Model Differentiation System; Chapter 12 Cross & Kalley: Induction of Differentiation of Human Intestinal Cell in Vitro; Chapter 13 Thalhammer & Cross: Proliferation.... [data incomplete]

Reviews

Intended for researchers studying cell growth, particularly human cell systems, this practical manual covers the measurement of cell proliferation, analysis of the transduction pathways for growth, and studies of growth cessation in cell differentiation and senescence. The conceptual underpinnings of each approach are provided, with details of procedures and guidelines for interpretation of results. Two chapters are retained and updated from the predecessor volume titled Cell Growth and Apoptosis. --SciTech Book News<br> Readers interested in an account that places cellular senescence at the epicenter of human aging and assembles as much evidence as possible to support that view will find a great deal to satisfy the in Cells, Aging, and Human Disease. --Science<br>

<br> Intended for researchers studying cell growth, particularly human cell systems, this practical manual covers the measurement of cell proliferation, analysis of the transduction pathways for growth, and studies of growth cessation in cell differentiation and senescence. The conceptual underpinnings of each approach are provided, with details of procedures and guidelines for interpretation of results. Two chapters are retained and updated from the predecessor volume titled Cell Growth and Apoptosis. --SciTech Book News<p><br> Readers interested in an account that places cellular senescence at the epicenter of human aging and assembles as much evidence as possible to support that view will find a great deal to satisfy the in Cells, Aging, and Human Disease. --Science<p><br>

""" ... is very useful for cell biologists, both newcomers and more experienced ones"" Cell Biology International"

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