Catharanthus roseus: Methods and Protocols

Author:   Vincent Courdavault ,  Sebastien Besseau
Publisher:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2505
ISBN:  

9781071623510


Pages:   319
Publication Date:   25 June 2023
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Catharanthus roseus: Methods and Protocols


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Author:   Vincent Courdavault ,  Sebastien Besseau
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2505
Weight:   0.640kg
ISBN:  

9781071623510


ISBN 10:   1071623516
Pages:   319
Publication Date:   25 June 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

RNA in situ hybridization on paraffin-sections to characterize the multicellular compartmentation of plant secondary metabolisms.- Imaging MS analysis in Catharanthus roseus.- Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle.- Sample preparation, data acquisition, and data analysis for 15N-labeled and non-labeled monoterpene indole alkaloids in Catharanthus roseus.- Studying iridoid transport in Catharanthus roseus by grafting.- Pictet-Spengler reaction for the chemical synthesis of strictosidine.- Implementation of a MS/MS spectral library for Monoterpene Indole Alkaloids.- Semi-Synthesis of Bis-Indole Alkaloid (–)-Melodinine K Enabled by a Combination of Biotransformation and Chemical Synthesis.- RNA-seq analysis towards monoterpene indole alkaloid biosynthetic pathway elucidation in Catharanthus roseus.- Predicting Monoterpene Indole Alkaloid related genes from expression data with artificial neural networks.- Discovery and characterization of oxidative enzymes in monoterpenoid indole alkaloid biosynthesis.- Ancestral sequence reconstruction for exploring alkaloid evolution.- Generating an EMS mutant population and rapid mutant screening by thin layer chromatography enables the studies of monoterpenoid indole alkaloids biosynthesis in Catharanthus roseus.- TARGETing transcriptional regulation in the medicinal plant Catharanthus roseus.- Identification and Characterization of Transcription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus.- From Methylome to Integrative Analysis of Tissue-Specificity.- Tagging and capture of prenylated CaaX-proteins from plant cell cultures.- EASI transformation protocol: An Agrobacterium-mediated transient transformation protocol for Catharanthus roseus seedlings.- A rapid and efficient vacuum-based Agroinfiltration protocol for transient gene overexpression in leaves of Catharanthus roseus.- Transient gene expression in Catharanthus roseus flower petals using agroinfiltration.- De novo shoot bud induction from the Catharanthus roseus leaf explants and Agrobacterium tumefaciens mediated technique to raise transgenic plants.- Agrobacterium-mediated in planta transformation in Periwinkle.

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