Overview

Capillary Electrophoresis (CE) provides researchers with the ability to separate and analyze very small amounts of DNA. CE offers important advantages over other available methods because of the speed and reproducibility of analysis. Further, the high sensitivity of detection and the ability to increase sample throughput with parallel analysis has led to the creation of a full range of techniques for the analysis of DNA molecules, from modified DNA-adducts and single strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids provides these analytical protocols, which can be used for detection and analysis of mutations and modifications to precise DNA loci through to entire genomes of organisms. Volume I broadly addresses instrumentation, signal detection and the capillary environment, as well as the integration of mass spectrometry and CE for the analysis of small oligonucleotides and modified nucleotides. It opens with a description of the basic CE instrumentation and discusses DNA sequencing by CE and the methods for the manufacture of microchip devices. Also covered are issues regarding the choices of separation media, and the fast separations of large DNA molecules and chromosomes using pulsed-field capillary electrophoresis. The book closes by offering choices for the analysis of small therapeutic oligonucleotides, nucleosides, ribonucleotides and DNA metabolism product by CE as well as those products arising from environmental and cellular damage through disease.

Full Product Details

Author:   Keith R. Mitchelson ,  Jing Cheng
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2001 ed.
Volume:   162
Dimensions:   Width: 15.20cm , Height: 2.90cm , Length: 22.90cm
Weight:   1.015kg
ISBN:  

9780896037793

ISBN 10:   0896037797
Pages:   484
Publication Date:   27 December 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Analytical Considerations for Capillary Electrophoresis.- Overview: The Application of Capillary Electrophoresis for DNA Polymorphism Analysis.- DNA Separation Mechanisms During Electrophoresis.- Purification Methods for Preparing Polymerase Chain Reaction Products for Capillary Electrophoresis Analysis.- Quantitative Measurements.- Microchip-Based Capillary Electrophoresis Systems.- DNA Sequencing by Capillary Array Electrophoresis.- 7 Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophoresis.- Capillary Matrix: Considerations for Capillary Electrophoresis.- Influence of Polymer Concentration and Polymer Composition on Capillary Electrophoresis of DNA.- Capillary Coatings.- Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis.- Sieving Matrix Selection.- Parameters Affecting Capillary Electrophoretic Separation of DNA.- Electrophoresis Matrix: Applications for DNA Fragment Sizing.- DNA Separation by Capillary Electrophoresis in Lyotropic Polymer Liquid Crystals.- Capillary Electrophoresis of DNA Fragments Using Poly(Ethylene Oxide) as a Sieving Material.- DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium.- 16 DNA Analysis Under Highly Denaturing Conditions in Bare Fused Silica Capillaries.- Capillary Electrophoresis with Glycerol as an Additive.- Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels.- Practical Applications for Large DNA Fragment Sizing and Fragment Collection.- Robust Field Inversion Capillary Electrophoretic Separation of Long DNA Fragments.- Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments.- Collection of Capillary Electrophoresis Fractions on a Moving Membrane.- Separation of Supercoiled DNA Using CapillaryElectrophoresis.- Practical Applications for Oligonucleotide and Nucleotide Analysis.- Quality Control of Nucleotides and Primers for PCR.- Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis.- Use of Capillary Electrophoresis for Concentration Analysis of Phosphorothioate Oligonucleotides.- Capillary Electrophoresis Separation of Ribonucleosides.- On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Capillaries.- Practical Applications for Analysis of Environmental Damage and Cellular Metabolism of DNA.- Capillary Electrophoresis-Mass Spectrometric Analysis of DNA Adducts.- Analysis of Environment-Induced DNA Damage by Capillary Electrophoresis.- Assay for DNA Damage Using Immunochemical Recognition and Capillary Electrophoresis.- Analysis of DNA Damage Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry.- Integration of Phosphodiesterase-Induced Degradation of Oligonucleotides with Capillary Polymer-Sieving Electrophoresis.- Capillary Electrophoresis of Urinary Normal and Modified Nucleosides of Cancer Patients.

Reviews

These volumes comprehensively and accurately present CE technology and techniques with an emphasis on providing an understanding of the theory and instrumentation (Vol. 1), as well as detailed examples of successful analytical protocols (both volumes) Chapters on the use of microchip and mass spectrometric detection are also included Although numerous experimental protocols can be found in Vol. 1, it is clear that the intent is to communicate the underlying theory and capability of CE for separations. Vol. 1 should be of interest to the novice as well as the expert. In Vol. 2, each of the 31 chapters present specific examples of the use of CE Nonetheless, these two volumes of the Methods in Molecular Biology series offer a representative and needed overview of the current theory and practice of CE of nucleic acids. These texts should prove very useful to both active investigators in this area and those seeking to learn more about the use and and capability of CE. - Clinical Chemistry Review for both volumes: This two-volume set of books comprises 64 chapters of protocols for the analysis of just about every form and size of nucleic acids that one can imagine. Thus it rates near an A for its comprehensiveness. - Analytical Chemistry

These volumes comprehensively and accurately present CE technology and techniques with an emphasis on providing an understanding of the theory and instrumentation (Vol. 1), as well as detailed examples of successful analytical protocols (both volumes)...Chapters on the use of microchip and mass spectrometric detection are also included...Although numerous experimental protocols can be found in Vol. 1, it is clear that the intent is to communicate the underlying theory and capability of CE for separations. Vol. 1 should be of interest to the novice as well as the expert. In Vol. 2, each of the 31 chapters present specific examples of the use of CE...Nonetheless, these two volumes of the Methods in Molecular Biology series offer a representative and needed overview of the current theory and practice of CE of nucleic acids. These texts should prove very useful to both active investigators in this area and those seeking to learn more about the use and and capability of CE. - Clinical Chemistry Review for both volumes: This two-volume set of books comprises 64 chapters of protocols for the analysis of just about every form and size of nucleic acids that one can imagine. Thus it rates near an A for its comprehensiveness. -Analytical Chemistry ...rates near an A for its comprehensiveness. - Analytical Chemistry

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