Overview

Capillary Electrophoresis Guidebook offers both newcomers and experienced research workers hands-on guidance to performing capillary electrophoresis. It provides sufficient practical advice to permit you to develop and optimize your own separations, along with extensive troubleshooting sections to overcome practical difficulties. The book contains operating instructions for standard commercially available instruments and includes guidelines for activities such as changing capillaries, method development, quantitative procedures, optimizing sensitivity, and the validation of methods. Review chapters written by leading experts discuss micellar electrokinetic capillary chromatography, capillary gel electrophoresis, advanced sampling techniques, and electrochromatography. Important application areas such as the analysis of proteins, peptides, amino acids, pharmaceuticals, chiral compounds, and nucleic acids are also treated.

Full Product Details

Author:   Kevin D. Altria
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1996 ed.
Volume:   52
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   0.686kg
ISBN:  

9780896033153

ISBN 10:   0896033155
Pages:   349
Publication Date:   02 November 1995
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

General Guidelines to the Operation of Capillary Electrophoresis Methods and Instrumentation.- Fundamentals of Capillary Electrophoresis Theory.- Standard Commercial Instrument Description.- Typical Operating Procedures.- Method Development/Optimization.- Quantitation Procedures.- Optimization of Precision in Quantitative Analysis.- Optimization of Sensitivity.- Method Validation.- Fraction Collection.- Troubleshooting.- Quick Guide to Running a Successful Separation.- Applications of Capillary Electrophoresis and Specific Technologies.- Micellar Electrokinetic Chromatography.- Capillary Gel Electrophoresis.- Chiral Separations by Capillary Electrophoresis.- Capillary Electrochromatography.- Application and Limits of Sample Stacking in Capillary Electrophoresis.- Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresis.- Application of Capillary Electrophoresis to Pharmaceutical Analysis.- Separation of Peptides and Protein Digests by Capillary Electrophoresis.- Additional Application Areas of Capillary Electrophoresis.

Reviews

The book is a very useful guidebook for CE practitioners as well as a reference book for researchers and graduate students. I highly recommend this book for libraries and individuals active in the field of CE. - FEBS Letters

The book is a very useful guidebook for CE practitioners as well as a reference book for researchers and graduate students. I highly recommend this book for libraries and individuals active in the field of CE.-FEBS Letters

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