Candida auris: Methods and Protocols

Author:   Alexander Lorenz
Publisher:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2517
ISBN:  

9781071624166


Pages:   342
Publication Date:   09 June 2022
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Candida auris: Methods and Protocols


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Author:   Alexander Lorenz
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2517
Weight:   0.874kg
ISBN:  

9781071624166


ISBN 10:   1071624164
Pages:   342
Publication Date:   09 June 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preface… Table of Contents… Contributing Authors… Part I Isolation and Identification of Candida auris 1. Isolation of Candida auris in Clinical Specimens  Anamika Yadav, Ashutosh Singh, and Anuradha Chowdhary  2. Candida auris Identification and Profiling by MALDI-ToF Mass Spectrometry  Alireza Abdolrasouli and Mark A. Fraser 3. Molecular Tools for Candida auris Identification and Typing Auke W. de Jong, Bert Gerrits van den Ende, and Ferry Hagen 4. qPCR Detection of Candida auris using the GPS™ CanAur MONODOSE dtec-qPCR Test Antonio Martínez-Murcia, Patricia Ros-Tárraga, Aaron Navarro, and Gema Bru Part II Testing Susceptibility of Candida auris to Antifungal Drugs 5. Susceptibility Testing of Candida auris Isolates Elizabeth M. Johnson and Andrew M. Borman 6. Deciphering Candida auris Paradoxical Growth Effect (Eagle Effect) in Response to Echinocandins Milena Kordalewska and David S. Perlin Part III Genetic Manipulation of Candida auris 7. An Optimized Electroporation Procedure for Genetic Transformation of Candida auris  Dalal Kasir, Marwan Osman, Monzer Hamze, Jean-Philippe Bouchara, Vincent Courdavault, and Nicolas Papon 8. Genetic Transformation of Candida auris via Homology-Directed Repair Using a Standard Lithium-Acetate Protocol Gustavo Bravo Ruiz and Alexander Lorenz 9. Downregulation of Essential Genes in the Fungal Pathogen Candida auris Kali R. Iyer, Sang Hu Kim, Nicole Robbins, and Leah E. Cowen Part IV Phenotypic and Biochemical Characterization of Candida auris 10. Cell Viability Assays for Candida auris   Jason L. Brown, Bryn Short, Alicia Ware, Leighann Sherry, Ryan Kean, and Gordon Ramage 11. Measuring Gene Expression via mRNA Abundance in Candida auris   Mohd Wasi, Remya Nair, Nitesh Kumar Khandelwal, and Rajendra Prasad 12. Gas Chromatography–Mass Spectrometry (GC-MS) Analysis of Candida auris Metabolites Bahgat Fayed, Ghalia Kohder, and Sameh S. M. Soliman 13. Isolation of Extracellular Vesicles from Candida auris  Daniel Zamith-Miranda, Lysangela R. Alves, Marcio L. Rodrigues, Leonardo Nimrichter, and Joshua D. Nosanchuk 14. Labelling of Candida auris Cell Walls to Examine Levels of PAMP Exposure by Flow Cytometry and Fluorescence Microscopy Chloe Pelletier and Alexander Lorenz 15. High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall Gillian Milne and Louise A. Walker Part V Bioinformatic Methods for Candida auris 16. Generating Complete Genome Assemblies of Candida auris Terrance Shea and Christina A. Cuomo 17. MycoSNP: A Portable Workflow for Performing Whole-Genome Sequencing Analysis of Candida auris Ujwal R. Bagal, John Phan, Rory M. Welsh, Elizabeth Misas, Darlene Wagner, Lalitha Gade, Anastasia P. Litvintseva, Christina A. Cuomo, and Nancy A. Chow 18. Bioinformatic Identification of ABC Transporters in Candida auris Atanu Banerjee, Poonam Vishwakarma, Naveen Kumar Meena, Andrew M. Lynn, and Rajendra Prasad Part VI Exploring Host-Pathogen Interactions of Candida auris 19. Examining Neutrophil-Candida auris Interactions with Human Neutrophils Ex Vivo Chad J. Johnson and Jeniel E. Nett 20. Modeling Candida auris Colonization on Porcine Skin Ex Vivo Chad J. Johnson, J. Z. Alex Cheong, Dhanansayan Shanmuganayagam, Lindsay R. Kalan, and Jeniel E. Nett 21. A Human Ex Vivo Skin Model to Study Candida auris Biofilms Dora E. Corzo-León, Catherine Mark, Donna M. MacCallum, and Carol A. Munro 22. In Vivo Skin Colonization/Decolonization Models for Candida auris Janet Herrada, Ahmed Gamal, Lisa Long, and Mahmoud A. Ghannoum 23. Use of Galleria mellonella Larvae to Study the Pathogenicity and Clonal Lineage-Specific Behaviors of the Emerging Fungal Pathogen Candida auris Andrew M. Borman 24. Drosophila melanogaster as a Rapid and Reliable In Vivo Infection Model to Study the Emerging Yeast Pathogen Candida auris Sebastian Wurster, Nathaniel D. Albert, and Dimitrios P. Kontoyiannis 25. An Immunocompromised Mouse Model of Candida auris Systemic Infection Janet Herrada, Kyle Roberts, Ahmed Gamal, Lisa Long, and Mahmoud A. Ghannoum 26. Mouse Gastrointestinal Colonization Model for Candida auris Stylianos C. Simantirakis and Donna M. MacCallum Subject Index List…

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