Overview

Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiasis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology.

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9781607611905

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<p>Aus den Rezensionen: <p><p> Dieses Buch ist f r eine klar definierte Gruppe von Lesern konzipiert ... also insbesondere Doktoranden und Postdoktoranden. In 18 Kapiteln beschreiben erfahrene Wissenschaftler sehr detailliert und nachvollziehbar Experimente aus unterschiedlichen Bereichen ... Eine kurze Einleitung erl utert ... den Hintergrund und die Fragestellungen, f r die Methoden insbesondere geeignet sind, sowie ihre Grenzen. Gro er Pluspunkt ... sind die am Ende jedes Kapitels aufgelisteten, spezifischen Anmerkungen zu einzelnen Punkten, die helfen sollen, Fehler zu vermeiden und ... informative und hilfreiche Erl uterungen zum Warum und Wie der Methode geben ... (Joachim Morschh user, in: BIOspektrum, 2009, Vol. 15, Issue 5, S. 581)

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