Overview

Calcium plays an important role in a wide variety of biological processes and is uniquely suited to act as a promoter or preventer of activities in biological systems. These two volumes provide the modern experimental techniques for studying calcium-binding proteins and their behaviour in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods, and many of the methods described can also be used in the study of proteins that do not bind calcium. Volume I of this two-volume set provides information on the role of calcium in intracellular secondary messenger activation mechanisms. It also provides a wealth of information about protein purification strategies, X-ray crystallography and other studies focused on specific calcium-binding proteins. By introducing the various classes of intra- and extra-cellular calcium-binding proteins and their mode of action, this first volume sets the stage and provides the necessary background for volume II. The second volume provides a vast array of modern experimental techniques that can be used to study the solution structure, stability, dynamics, calcium-binding properties and biological activity of calcium-binding proteins in general. Together, these two volumes provide all that a researcher needs to conduct research on calcium-binding proteins.

Full Product Details

Author:   Hans J. Vogel
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   172
Dimensions:   Width: 15.50cm , Height: 2.50cm , Length: 23.30cm
Weight:   0.765kg
ISBN:  

9780896036888

ISBN 10:   089603688
Pages:   340
Publication Date:   02 January 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

and Reviews.- Calcium-Binding Proteins.- Calcium.- Crystal Structure of Calpain and Insights into Ca2+-Dependent Activation.- The Multifunctional S100 Protein Family.- Ca2+Binding to Proteins Containing ?-Carboxyglutamic Acid Residues.- The Caseins of Milk as Calcium-Binding Proteins.- Calcium-Binding Proteins: Case Studies.- Preparation of Recombinant Plant Calmodulin Isoforms.- Isolation of Recombinant CardiacTroponin C.- Skeletal Muscle Troponin C.- Purification of Recombinant Calbindin D9k.- S100 Proteins.- Cadherins.- ?-Lactalbumin and (Calcium-Binding) Lysozyme.- Recombinant Annexin II Tetramer.- Purification and Characterization of ALG-2 A Novel Apoptosis-Linked Ca2+-Binding Protein.- Crystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain VI of Calpain.- Neurocalcin.- Crystallization and Structure-Function of Calsequestrin.- Use of Fluorescence Resonance Energy Transfer to Monitor Ca2+-Triggered Membrane Docking of C2 Domains.- Ca2+-Binding Mode of the C2A-Domain of Synaptotagmin.- Study of Calcineurin Structure by Limited Proteolysis.

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