Overview

Calcium plays an important role in a wide variety of biological processes and is uniquely suited to act as a promoter or preventer of activities in biological systems. These two volumes provide the modern experimental techniques for studying calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods, and many of the methods described can also be used in the study of proteins that do not bind calcium. Volume II of this two volume set provides a vast array of modern experimental techniques that can be used to study the solution structure, stability, dynamics, calcium-binding properties and biological activity of calcium-binding proteins in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, particular attention is paid to modern NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, as well as fluorescence methods to study the distribution of calcium and calcium binding proteins in cells. In addition, studies of their ligand binding properties and their distribution in cells are included. Volume I of this two-volume set provides information on the role of calcium in intracellular secondary messenger activation mechanisms. It also provides a wealth of information about protein purification strategies, X-ray crystallography and other studies focused on specific calcium-binding proteins. By introducing the various classes of intra- and extra-cellular calcium-binding proteins and their mode of action, this first volume sets the stage and provides the necessary background for Volume II. Together, these two volumes provide all that a researcher needs to conduct research on calcium-binding proteins.

Full Product Details

Author:   Hans J. Vogel
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2002 ed.
Volume:   173
Dimensions:   Width: 15.50cm , Height: 2.80cm , Length: 23.50cm
Weight:   1.780kg
ISBN:  

9780896036895

ISBN 10:   0896036898
Pages:   415
Publication Date:   24 January 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Methods and Techniques to Study.- Quantitative Analysis of Ca2+-Binding by Flow Dialysis.- Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators.- Deconvolution of Calcium-Binding Curves.- Absorption and Circular Dichroism Spectroscopy.- Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins.- Steady-State Fluorescence Spectroscopy.- Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins.- Surface Plasmon Resonance of Calcium-Binding Proteins.- Differential Scanning Calorimetry.- Isothermal Titration Calorimetry.- Multiangle Laser Light Scattering and Sedimentation Equilibrium.- Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in their Interactions with Regulatory Targets.- Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry.- Synthetic Calcium-Binding Peptides.- Proteolytic Fragments of Calcium-Binding Proteins.- Electron Magnetic Resonance Studies of Calcium-Binding Proteins.- Cadmium-113 and Lead-207 NMR Spectroscopic Studies of Calcium-Binding Proteins.- Calcium-43 NMR of Calcium-Binding Proteins.- Exploring Familial Relationships Using Multiple Sequence Alignment.- Structure Determination by NMR.- Protein Structure Calculation from NMR Data.- Shape and Dynamics of a Calcium-Binding Protein Investigated by Nitrogen-15 NMR Relaxation.- The Use of Dipolar Couplings for the Structure Refinement of a Pair of Calcium-Binding EGF Domains.- Vector Geometry Mapping.- Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography.- Enzymatic Assays to Compare Calmodulin Isoforms, Mutants, and Chimeras.- Gene Expression inTransfected Cells.- Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins.- Studying the Spatial Distribution of Ca2+-Binding Proteins.

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