C. elegans: Methods and Applications

Author:   David Biron ,  Gal Haspel
Publisher:   Humana Press Inc.
Edition:   2nd ed. 2015
Volume:   1327
ISBN:  

9781493928415


Pages:   252
Publication Date:   02 October 2015
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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C. elegans: Methods and Applications


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Author:   David Biron ,  Gal Haspel
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2015
Volume:   1327
Dimensions:   Width: 17.80cm , Height: 2.00cm , Length: 25.40cm
Weight:   7.035kg
ISBN:  

9781493928415


ISBN 10:   1493928414
Pages:   252
Publication Date:   02 October 2015
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Library Construction for Mutation Identification by Whole-Genome Sequencing.- Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes.- Genetic Methods for Cellular Manipulations in C. elegans.- A Fusion PCR Method for Expressing Genetic Tools in C. elegans.- Transposon Assisted Genetic Engineering with Mos1 Mediated Single-copy Insertion (MosSCI).- Creating Genome Modifications in C. elegans using the CRISPR/Cas9 System.- Observing and Quantifying Fluorescent Reporters.- Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior.- Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans.- High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegans.- Electron Tomography Methods for C. elegans.- Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans.- Tracking Single C. elegans using a USB Microscope on a Motorized Stage.- An Imaging System for C. elegans Behavior.- A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans dauer Larvae.- Sampling and Isolation of C. elegans from the Natural Habitat.- A Primer on Prototyping.- A Primer on Quantitative Modeling.

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