Overview

Biomolecular Interactions: Part A, Volume 166, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics in cell biology. Each chapter is written by an international board of authors.

Full Product Details

Author:   Arun K. Shukla (Indian Institute of Technology, Kanpur, India)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Weight:   0.450kg
ISBN:  

9780128233511

ISBN 10:   0128233516
Pages:   368
Publication Date:   27 October 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1: Measuring the rapid kinetics of receptor-ligand interactions in live cells using NanoBRET 2: Evaluating functional ligand-GPCR interactions in cell-based assays 3: Assays for detecting arrestin interaction with GPCRs 4: BRET-based assay to specifically monitor ß2AR/GRK2 interaction and ß-arrestin2 conformational change upon ßAR stimulation 5: Cannabinoid Receptor CB1 and CB2 Interacting Proteins: Techniques, Progress and Perspectives 6: Purinergic GPCR transmembrane residues involved in ligand recognition and dimerization 7: Nanobodies as Sensors of GPCR Activation and Signaling 8: Confocal and TIRF microscopy based approaches to visualize arrestin trafficking in living cells 9: Strategies for targeting cell surface proteins using multivalent conjugates and chemical biology 10: Identifying Plasmodium falciparum receptor activation using bioluminescence resonance energy transfer (BRET)-based biosensors in HEK293 cells 11: Methods for Binding Analysis of Small GTP-Binding Proteins with their Effectors 12: Protein-protein interactions in the brain: protocol for preparing rodent brain tissue for mass spectrometry-based quantitative- and phospho- proteomics analysis 13: Protein-Protein Interactions at a glance: protocols for the visualization of biomolecular interactions 14: Interactions between Noncoding RNAs as Epigenetic Regulatory Mechanisms in Cardiovascular Diseases

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Author Information

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

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