Overview

This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins. Although centered on the development of bioconjugates rather than their application, most protocols also show the conjugation of the targeting vehicle to a diagnostic or therapeutic entity, with the end-product most often being an antibody-drug conjugate, an optical probe, a nanomedicine, or a radiopharmaceutical. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Bioconjugation: Methods and Protocols is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery.

Full Product Details

Author:   Sam Massa ,  Nick Devoogdt
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2033
Weight:   0.633kg
ISBN:  

9781493996568

ISBN 10:   1493996568
Pages:   317
Publication Date:   14 August 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Site-Specific Conjugation of Thiol-Reactive Cytotoxic Agents to Non-Native Cysteines of Engineered Monoclonal Antibodies             Stephan Dickgiesser, Roland Kellner, Harald Kolmar, and Nicolas Rasche   2. Application of Next Generation Maleimides (NGMs) to Site-Selective Antibody Conjugation             Maurício Morais, Nafsika Forte, Vijay Chudasama, and James R. Baker   3. Lysine Bioconjugation on Native Albumin with a Sulfonyl Acrylate Reagent             Maria J. Matos, Gonzalo Jiménez-Osés, and Gonçalo J.L. Bernardes   4. Engineering Dual-Variable-Domains for the Generation of Site-Specific Antibody-Drug Conjugates             Alex R. Nanna and Christoph Rader   5. Efficient Production of Homogeneous Lysine-Based Antibody Conjugates Using Microbial Transglutaminase             Jared L. Spidel and Earl F. Albone   6. Engineering Antibodies with C-Terminal Sortase-Mediated Modification for Targeted Nanomedicine             Rania A. Hashad, Jaclyn L. Lange, Natasha C.W. Tan, Karen Alt, and Christoph E. Hagemeyer   7. CRISPR/Cas9-Mediated Genetic Engineering of Hybridomas for Creation of Antibodies that Allow Site-Specific Conjugation             Makan Khoshnejad, Jacob S. Brenner, Hamideh Parhiz, and Vladimir R. Muzykantov   8. Site-Specific Modification of Proteins via Trypsiligase             Sandra Liebscher and Frank Bordusa   9. Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovative Bioconjugation             Geert-Jan Graulus, Duy Tien Ta, Huong Tran, Rebekka Hansen, Brecht Billen, Erik Royackers, Jean-Paul Noben, Nick Devoogdt, Serge Muyldermans, Wanda Guedens, and Peter Adriaensens   10. Site-Specific Bioconjugation Using SMARTagâ Technology: A Practical and Effective Chemoenzymatic Approach to Generate Antibody-Drug Conjugates             Junjie Liu, Robyn M. Barfield, and David Rabuka   11. Protein Labeling and Bioconjugation Using N-Myristoyltransferase             Karin F.K. Ejendal, Julia G. Fraseur, and Tamara L. Kinzer-Ursem   12. One-Step Fluorescent Protein Labeling by Tubulin Tyrosine Ligase             Dominik Schumacher, Heinrich Leonhardt, Christian P.R. Hackenberger, and Jonas Helma   13. Enzymatically Catalyzed Radiofluorination of Biomolecules             Christopher R. Drake, Michael J. Evans, and Henry F. VanBrocklin   14. Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransferase             Yi Zhang, Shelby Auger, Jonas V. Schaefer, Andreas Plückthun, and Mark D. Distefano   15. Homogeneous Antibody-Drug Conjugates via Glyco-Engineering             Feng Tang, Wei Shi, and Wei Huang   16. Labeling Proteins at Site-Specifically Incorporated 5-Hydroxytryptophan Residues Using a Chemoselective Rapid Azo-Coupling Reaction             Partha Sarathi Addy, Sarah B. Erickson, James S. Italia, and Abhishek Chatterjee   17. Facile Site-Specific Multi-Conjugation Strategies in Recombinant Proteins Produced in Bacteria             Hannes Merten, Jonas V. Schaefer, Fabian Brandl, Uwe Zangemeister-Wittke, and Andreas Plückthun   18. Site-Specific Photocrosslinking to Immunoglobulin G Using Photoreactive Antibody-Binding Domains             Fabiana Zappala and Andrew Tsourkas   19. SpyTag-SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo             Xiao-Wei Wang and Wen-Bin Zhang   20. FN3 Protein Conjugates for Cancer Diagnosis and Imaging Studies             Arutselvan Natarajan and Lotfi Abou-Elkacem

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