Overview

This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.

Full Product Details

Author:   Natalie Verstraeten ,  Jan Michiels
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2357
Weight:   0.588kg
ISBN:  

9781071616239

ISBN 10:   1071616234
Pages:   292
Publication Date:   01 October 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

PART I: Introduction 1.     Studying [NV1] Bacterial Persistence: Established Methods And Current Advances Elen Louwagie, Laure Verstraete, Jan Michiels, and Natalie Verstraeten PART II: Quantification and enrichment of persister cells 2.     Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases Enea Maffei, Cinzia Fino, and Alexander Harms 3.     A Robust Method For Generating, Quantifying, and Testing Large Numbers of Escherichia coli persisters Silke R. Vedelaar, Jakub L. Radzikowski, and Matthias Heinemann 4.     Enrichment of Persister Cells through β-lactam-induced Filamentation and Size Separation Etthel Windels, Bram Van den Bergh, and Jan Michiels 5.     Methods for Enrichment of Bacterial Persister Populations For Phenotypic Screens And Genomic Studies Samantha Adikari, Elizabeth Hong-Geller, and Sofiya Micheva-Viteva PART III : Single-cell analysis of persister cells 6.     Observing Bacterial Persistence at Single-Cell Resolution Emma Dawson, Emrah Şimşek, and Minsu Kim 7.     Phenotypic Characterization of Antibiotic Persisters at the Single-Cell Level: From Data Acquisition to Data Analysis Nathan Fraikin, Laurence Van Melderen, and Frédéric Goormaghtigh 8.     Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation Stefany Moreno-Gámez, Alma Dal Co, Simon van Vliet, and Martin Ackermann 9.     Counting Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence Allison M. Murawski, Katherine Rittenbach, Christina J. DeCoste, Gary Laevsky, and Mark P. Brynildsen PART IV: Omics approaches in persistence research 10.  Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods Bork A. Berghoff 11.  Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa Pablo Manfredi, Isabella Santi, Enea Maffei, Emmanuelle Lezan, Alexander Schmidt, and Urs Jenal 12.  The use of Experimental Evolution to Study The Response of Pseudomonas aeruginosa to Single or Double Antibiotic Treatment Isabella Santi, Pablo Manfredi, Urs Jenal PART V: Persister cell resuscitation and eradication 13.  Detecting persister awakening determinants Dorien Wilmaerts, Jan Michiels, and Natalie Verstraeten 14.  Monitoring Persister Resuscitation with Flow Cytometry Sayed Golam Mohiuddin and Mehmet A. Orman 15.  Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus persisters Ashelyn E. Sidders, Lauren C. Radlinski, Sarah E. Rowe, and Brian P. Conlon PART VI: Cellular and animal model systems for studying persistence 16.  In vitro Models for the Study of the Intracellular Activity Of Antibiotics Frédéric Peyrusson, Tiep K. Nguyen, Julien M. Buyck, Sandrine Lemaire, Gang W. Wang, Cristina Seral, Paul M. Tulkens, and Van Bambeke 17.  Analysis of Salmonella Persister Population Sizes, Dynamics Of Gut Luminal Seeding, And Plasmid Transfer In Mouse Models Of Salmonellosis Erik Bakkeren, Joshua P. M. Newson, and Wolf-Dietrich Hardt 18.  Studying Antibiotic Persistence During Infection Charlotte Michaux, Severin Ronneau, and Sophie Helaine

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