Overview

For both volumes: Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

Full Product Details

Author:   Shaying Zhao ,  Marvin Stodolsky
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2004
Volume:   255
Dimensions:   Width: 15.20cm , Height: 2.10cm , Length: 22.90cm
Weight:   0.561kg
ISBN:  

9781617372889

ISBN 10:   1617372889
Pages:   360
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

BAC Library Construction.- Construction of Small Genome BAC Library for Functional and Genomic Applications.- Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging.- Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions.- Purification of BAC DNA.- Hybridization-Based Selection of BAC Clones.- Applications of Interspersed Repeat Sequence Polymerase Chain Reaction.- BAC Mapping Using Fluorescence In Situ Hybridization.- High-Throughput BAC Fingerprinting.- BAC End Sequencing.- Radiation Hybrid Mapping With BAC Ends.- Shotgun Library Construction for DNA Sequencing.- Rolling Circle Amplification for Sequencing Templates.- Transposon-Mediated Sequencing.- Pyrosequencing.- Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions.- Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions.- Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers.- BAC Finishing Strategies.- Using the TIGR Assembler in Shotgun Sequencing Projects.- Finishing “Working Draft” BAC Projects by Directed Sequencing With ThermoFidelase and Fimers.- Optimized Multiplex Polymerase Chain Reaction.- Assembly of DNA Sequencing Data.- Sequence Finishing.- Quality Assessment of Finished BAC Sequences.

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