Overview

For both volumes: Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

Full Product Details

Author:   Shaying Zhao ,  Marvin Stodolsky
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   256
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   0.672kg
ISBN:  

9780896039896

ISBN 10:   0896039897
Pages:   326
Publication Date:   04 March 2004
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Use of BAC End Sequences for SNP Discovery.- Exon Trapping for Positional Cloning and Fingerprinting.- Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column.- BAC Microarray-Based Comparative Genomic Hybridization.- Large DNA Transformation in Plants.- Retrofitting BACs With a Selectable Marker for Transfection.- BAC Modification Using a RecA Expressing Shuttle Vector System.- Bacterial Artificial Chromosome Engineering.- ET Recombination.- BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes.- Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes.- Generation of BAC Transgenic Mice.- BAC Rescue.- Herpesviruses.- Cloning of ?-Herpesvirus Genomes as Bacterial Artificial Chromosomes.- Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors.- Mutagenesis of Viral BACs With Linear PCR Fragments (ET Recombination).- Mutagenesis of Herpesvirus BACs by Allele Replacement.- Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies.- Protective DNA Vaccination by Particle Bombardment Using BAC DNA Containing a Replication-Competent, Packaging-Defective Genome of Herpes Simplex Virus Type I.

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions