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OverviewArabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides an introduction to most of the key techniques required for the use of Arabidopsis as an experimental system. It gives a basic introduction to the optimal growth conditions and genetic resources available for Arabidopsis, how this material should be handled, maintained and used. Individual chapters describe strategies for the identification, mapping (using multi-marker lines and recombinant inbreds), and characterisation of different mutants by microscopy, molecular cytogenetics and gene expression analysis. Different cloning strategies, using transposons, T-DNA and map position are described in detail. Sequencing of the Arabidopsis genome will be completed in 2000 and bioinformatics are of key importance; the tools that are available and where they can be found on the Web are presented. Full Product DetailsAuthor: Zoe Wilson (Plant Science Division, School of Biological Sciences, University of Nottingham)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 223 Dimensions: Width: 16.00cm , Height: 2.10cm , Length: 24.10cm Weight: 0.638kg ISBN: 9780199635658ISBN 10: 019963565 Pages: 296 Publication Date: 06 January 2000 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Out of Print Availability: In Print  Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock. Table of ContentsGrowth, maintenance and use of Arabidopsis genetic resources Preservation and handling of stock centre clones Genetic mapping using recombinant inbred lines Arabidopsis mutant characterisation: microscopy, mapping and gene expression analysis Classical and molecular cytogenetics of Arabidopsis Tissue culture, transformation and transient gene expression in Arabidopsis Transposon and T-DNA mutagenesis Map based cloning in Arabidopsis YACs, BACs, cosmids and nucleotide sequences Web-based bioinformatic tools for Arabidopsis researchersReviewsCovers the key techniques for conducting experiments with Arabidopsis, long acknowledged to be the ideal molecular genetic tool for the analysis of development in higher plants. Covers optimal growth conditions, maintenance, available genetic resources, strategies and methodologies for the identification, mapping (using multi-marker liens and recombinant inbreds), and characterization of different mutants by microscopy, molecular cytogenetics, and gene expression analysis. Also covers different cloning approaches, using transposons, T-DNA and physical map positions, as well as the bioinformatics tools and their use for Arabidopsis research. Contributors consist of university-affiliated and industrial researchers, primarily from Britain. --SciTech Book News<br> <br> Covers the key techniques for conducting experiments with Arabidopsis, long acknowledged to be the ideal molecular genetic tool for the analysis of development in higher plants. Covers optimal growth conditions, maintenance, available genetic resources, strategies and methodologies for the identification, mapping (using multi-marker liens and recombinant inbreds), and characterization of different mutants by microscopy, molecular cytogenetics, and gene expression analysis. Also covers different cloning approaches, using transposons, T-DNA and physical map positions, as well as the bioinformatics tools and their use for Arabidopsis research. Contributors consist of university-affiliated and industrial researchers, primarily from Britain. --SciTech Book News<br> Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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