Overview

​Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production.  Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

Full Product Details

Author:   Mohamed Al-Rubeai
Publisher:   Springer International Publishing AG
Imprint:   Springer International Publishing AG
Edition:   Softcover reprint of the original 1st ed. 2015
Volume:   9
Dimensions:   Width: 15.50cm , Height: 3.90cm , Length: 23.50cm
Weight:   1.567kg
ISBN:  

9783319354521

ISBN 10:   3319354523
Pages:   763
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preface Chapter 1: Cell line development Hansjoerg Hauser Chapter 2: Transient recombinant protein expression in mammalian cells Volker Jäger, Konrad Büssow, Thomas Schirrmann Chapter 3: Hybridoma technology for the in vitro production of therapeutic antibodies Susan McDonnell Chapter 4: Bioreactors for mammalian cells                                                 R. Pörtner Chapter 5: Mass Transfer and Mixing Across the Scales in Animal Cell Culture Alvin W Nienow Chapter 6: Hydrodynamic Damage to Animal Cells Jeffrey J Chalmers and Ningning Ma Chapter 7: Monitoring of cell culture Sebastian Schwamb, Robert Puskeiler, Philipp Wiedemann Chapter 8: Serum and protein free media Michael Butler Chapter 9: Glycosylation in Cell Culture Maureen Spearman and Michael Butler Chapter 10: Modelling of mammalian cell cultures Nishikant P. Shirsat, Niall J. English, Brian Glennon and Mohamed Al-Rubeai Chapter 11: Mammalian Cell Line Selection Strategies for High-Producers Darrin Kuystermans and Mohamed Al-Rubeai Chapter 12: Building a Cell Culture Process with Stable Foundations: Searching for Certainty in an Uncertain World Peter M O’Callaghan and Andrew J Racher Chapter 13: Perfusion Processes Veronique Chotteau Chapter 14 : Single-use bioreactors for animal and human cells Stephan C. Kaiser, Matthias Kraume, Dieter Eibl, and Regine Eibl Chapter 15 : An Overview of Cell Culture Engineering for the Insect Cell-Baculovirus Expression Vector System (BEVS) Laura A. Palomares, Mauricio Realpe and Octavio T. Ramirez Chapter 16 : Metabolic flux analysis: a powerful tool in animal cell culture João V. Sá, Tiago M. Duarte, Manuel J. T. Carrondo, Paula M. Alves, Ana P. Teixeira Chapter 17 : Cell immobilization for the production of viral vaccines Ana Carina Silva, António Roldão, Ana Teixeira, Paulo Fernandes, Marcos F.Q. Sousa, Paula M. Alves Chapter 18 : Cell Engineering for Therapeutic Protein Production Eric Baek, Che Lin Kim, Jin Hyoung Park, and Gyun Min Lee Chapter 19 : Proteomics in Cell Culture: From genomics to combined ‘omics for cell line engineering and bioprocess development Kelley Heffner, Christian Kaas, Amit Kumar, Deniz Baycin-Hizal, Michael Betenbaugh Chapter 20 : Metabolomics in animal cell culture Miroslava Čuperlović-Culf Chapter 21 : Process Analytical Technology and Quality-by-Design for Animal Cell Culture Stephen Craven and Jessica Whelan Chapter 22 : Biosafety recommendations on the handling of animal cell cultures Philippe Herman and Katia Pauwels Chapter 23 : Biopharmaceutical products from animal cell culture Darrin Kuystermans and Mohamed Al-Rubeai Index  

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