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OverviewThis detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects. Full Product DetailsAuthor: Peter Scheiffele , Oriane MaugerPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 1st ed. 2022 Volume: 2537 Weight: 0.889kg ISBN: 9781071625200ISBN 10: 1071625209 Pages: 354 Publication Date: 28 July 2022 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of Contents1. Alternative Splicing in Human Biology and Disease Daniel Jutzi and Marc-David Ruepp 2. Targeting Alternative Splicing for Therapeutic Interventions Jessica L. Centa and Michelle L. Hastings 3. An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions Giulia Di Bartolomei and Peter Scheiffele 4. FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis Zicheng Wei, Yuxin Qin, Gordon Fishell, and Boxing Li 5. Quantitative Measurement of Alternatively Spliced RNA Isoform Levels Zhelin Jeff Li and Sika Zheng 6. Analysis of Splicing Regulation by Third-Generation Sequencing Eric Allemand and Fabrice Ango 7. Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework André Gohr, Federica Mantica, Antonio Hermoso-Pulido, Javier Tapial, Yamile Márquez, and Manuel Irimia 8. Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data Guillermo E. Parada and Martin Hemberg 9. Functional Annotation of Custom Transcriptomes Fursham Hamid, Kaur Alasoo, Jaak Vilo, and Eugene Makeyev 10. Design, Labeling, and Application of Probes for RNA smFISH Ewa Piskadlo, Bastian Th. Eichenberger, Luca Giorgetti, and Jeffrey A. Chao 11. BaseScope™ Approach to Visualize Alternative Splice Variants in Tissue Alexandra Bunda and Arturo Andrade 12. In Situ Imaging of mRNA Splicing Variants by SpliceRCA Xiaojun Ren and Jinghong Li 13. Two-Color Fluorescent Reporters for Analysis of Alternative Splicing John A. Calarco and Pallavi P. Pilaka 14. Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry Alexander Schmidt and Dietmar Schreiner 15. Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy Sébastien Campagne, Tebbe de Vries, and Frédéric H-T. Allain 16. Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention Anna M. Scarborough, Ashwin Govindan, and Nicholas K. Conrad 17. Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins Andrea B. Eberle and Oliver Mühlemann 18. Probing Liquid-Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo Stephanie Heinrich and Maria Hondele 19. Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals François Halloy, Paulina Ćwiek, Jonathan Hall, and Daniel SchümperliReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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