Overview

This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects.

Full Product Details

Author:   Tim Bartels
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1948
Weight:   0.801kg
ISBN:  

9781493991235

ISBN 10:   149399123
Pages:   289
Publication Date:   18 February 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons.- Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue.- Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons.- Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform.- Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed Fibrils.- Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection.- Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers.- Studying α-Synuclein Conformation by Intact-Cell Crosslinking.- C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques.- Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein.- CircularDichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes.- Yeast-Based Screens to Target Alpha-Synuclein Toxicity.- Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance Spectroscopy.- Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers.- Monitoring α-Synuclein Proteotoxicity in Drosophila Models.- Detection of α-Synuclein in Biological Samples using Mass Spectrometry.- Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation.- In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical Neurons.- Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy.- Expression and Purification of Untagged α-Synuclein.- Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson’s Disease.

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