Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics

Author:   William S. M. Wold ,  Ann E. Tollefson
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 2nd ed. 2007
Volume:   131
ISBN:  

9781617378355


Pages:   362
Publication Date:   10 November 2010
Format:   Paperback
Availability:   In Print   Availability explained
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Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics


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Author:   William S. M. Wold ,  Ann E. Tollefson
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 2nd ed. 2007
Volume:   131
Dimensions:   Width: 15.20cm , Height: 2.10cm , Length: 22.90cm
Weight:   0.555kg
ISBN:  

9781617378355


ISBN 10:   1617378356
Pages:   362
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Analysis of the Efficiency of Adenovirus Transcription.- The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains.- Preparation of Soluble Extracts From Adenovirus-Infected Cells for Studies of RNA Splicing.- In Vitro Methods to Study RNA Interference During an Adenovirus Infection.- Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ.- Study of Nucleolar Localization of Adenovirus Core Proteins.- Analysis of Adenovirus Infections in Synchronized Cells.- Co-Immunoprecipitation of Protein Complexes.- Chromatin Immunoprecipitation to Study the Binding of Proteins to the Adenovirus Genome In Vivo.- Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays.- Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry.- The Use of Cell Microinjection for the In Vivo Analysis of Viral Transcriptional Regulatory Protein Domains.- Determination of the Transforming Activities of Adenovirus Oncogenes.- Human Adenovirus Type 12.- Measurement of Natural-Killer Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells.- A Flow Cytometric Assay for Analysis of Natural-Killer Cell-Mediated Cytolysis of Adenovirus-Transformed Cells.- Large-Scale Purification and Crystallization of Adenovirus Hexon.- Synthesis and Assay of Recombinant Adenovirus Protease.- Assay for the Adenovirus Proteinase.- Cofactors of the Adenovirus Proteinase.- Characterization of the Adenovirus Fiber Protein.- Phylogenetic Analysis of Adenovirus Sequences.- Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies.

Reviews

...recommend it to investigators working in the area of viral-host interactions. -Doody's Book Review, Weighted Numerical Score: 70 - 3 Stars Reviews of the First edition: This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them...a very comprehensive methods book that will be a laboratory standard for years to come. - Microbiology Today ...this book constitutes an excellent reference text ...this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses. -FEBS Letters


...recommend it to investigators working in the area of viral-host interactions. -Doody's Book Review, Weighted Numerical Score: 70 - 3 Stars Reviews of the First edition: This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them...a very comprehensive methods book that will be a laboratory standard for years to come. - Microbiology Today ...this book constitutes an excellent reference text ...this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses. -FEBS Letters


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