Adenovirus Methods and Protocols: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models

Author:   William S. M. Wold ,  Ann E. Tollefson
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 2nd ed. 2007
Volume:   130
ISBN:  

9781617376696


Pages:   242
Publication Date:   10 November 2010
Format:   Paperback
Availability:   In Print   Availability explained
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Adenovirus Methods and Protocols: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models


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Full Product Details

Author:   William S. M. Wold ,  Ann E. Tollefson
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 2nd ed. 2007
Volume:   130
Dimensions:   Width: 15.20cm , Height: 1.40cm , Length: 22.90cm
Weight:   0.381kg
ISBN:  

9781617376696


ISBN 10:   1617376698
Pages:   242
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Manipulation of Early Region 4.- Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants.- Construction of Adenovirus Type 5 Early Region 1 and 4 Virus Mutants.- Construction of Mouse Adenovirus Type 1 Mutants.- Generation of Recombinant Adenovirus Using the Escherichia coli BJ5183 Recombination System.- Production and Release Testing of Ovine Atadenovirus Vectors.- Construction of Capsid-Modified Recombinant Bovine Adenovirus Type 3.- Adenovirus Capsid Chimeras.- Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis.- Evaluating Apoptosis in Tumor Cells Infected With Adenovirus Expressing p53 Tumor Suppressor Gene.- Growth and Purification of Enteric Adenovirus Type 40.- Immunocompetent, Semi-Permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses.- Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors.- A Real-Time PCR Method to Rapidly Titer Adenovirus Stocks.- Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR.- Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins.- Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs.- Preparation and Titration of CsCl-Banded Adenovirus Stocks.

Reviews

...provdes some good advice on using and growing adenovirus vectors. -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars Reviews of the First edition: This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them...a very comprehensive methods book that will be a laboratory standard for years to come. -Microbiology Today ...this book constitutes an excellent reference text ...this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses. -FEBS Letters


...provdes some good advice on using and growing adenovirus vectors. -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars Reviews of the First edition: This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them...a very comprehensive methods book that will be a laboratory standard for years to come. -Microbiology Today ...this book constitutes an excellent reference text ...this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses. -FEBS Letters


...provdes some good advice on using and growing adenovirus vectors. -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars Reviews of the First edition: This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them...a very comprehensive methods book that will be a laboratory standard for years to come. -Microbiology Today ...this book constitutes an excellent reference text ...this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses. -FEBS Letters


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