Overview

Microarray technology is more accessible than ever, and an ever-widening field of scientists is using this technology. However, the manufacture, experimental design, and analysis of microarrays are not always straightforward, and researchers new to the field run into technical and theoretical roadblocks that can hinder progress with this powerful new technology. This text addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology.

Full Product Details

Author:   Eric M. Blalock
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2003 ed.
Dimensions:   Width: 15.50cm , Height: 2.50cm , Length: 23.50cm
Weight:   0.784kg
ISBN:  

9781402074721

ISBN 10:   1402074727
Pages:   347
Publication Date:   31 July 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

1 Slide Coating And DNA immobilization Chemistries.- Glass Properties.- Glass Cleaning.- Slide Coating Chemistries.- Dna Immobilization Chemistries.- Surface Analysis Methods.- Summary.- References.- 2 Diagnostic Oligonucleotide Micro Arrays For Microbiology.- Scheme of The Experimental Approach.- Sources of Variation.- Establishment of a Sequence Database.- Oligo Length And Melting Temperature (Tm); Designing Oligo Sets Tuned To Work Together.- Oligo Set Design.- Choice Of Oligo/Surface Binding Chemistry.- Array Printing.- Target Preparation.- Hybridisation.- Scanning.- Data Analysis.- Applications In Microbial Identification.- Acknowledgements.- Links Related To Dlagnostic Microbial Microarrays.- Reference List.- 3 Printing Technologies And Microarray Manufacturing Techniques: Making The Perfect Microarray.- Microarray Manufacturing.- Comparing Printing Technologies.- Conclusions.- Acknowledgments.- Reference List.- 4 Arrays For The Masses — Setting Up a Microarray Core Facility.- Preface.- Hedco/Oregon Cancer Institute Spotted Microarray Core at Ohsu.- Core Set-Up.- Array Printing.- The Printer.- Slides.- Amplification.- Printing The Array.- Hybridization.- Scanner.- Other Equipment.- Conclusion.- References.- 5 Microarraydatanormalization: The Art And Science Of Overcoming Technical Variance To Maximize The Detection Of Biologic Variance.- Normalization: Correcting For Technical Variance In Order To Study Biological Variation.- Single Channel Data Normalizations.- Normalizations Of Two-Channel Data.- The Role Of Experimental Design In The Removal Of Technical Variance.- Gene-Specific Normalizations And Clustering.- References.- 6 Experimental Design And Data Analysis.- Measuring RNA.- Fold Change Significance.- Variance.- Experimental Design.- Variance andFold-Change.- Affymetrix Data.- Working With More Than Two Groups.- Functional Grouping.- Using Excel.- Acknowledgements.- References.- 7 Microarray Experiment Design And Statistic Analysis.- Designing Amicroarray Experiment.- General Procedures For Statistical Analysis Of Microarray Data.- Multiple Hypothesis Testing In Microarray Experiments.- Methods Based On P Value Adjustment.- Analysis Of Variance.- Summary Of The Chapter.- Some Useful Online Sources For Microarray Analysis.- References.- 8 Strategies For Clustering, Classifying Integrating, Standardizing And Visualizing Microarray Gene Expression Data.- Microarray Gene Expression Matrix.- Distance Functions.- Unsupervised Analysis And Clustering Of Microarray Data.- Methods For Validating Unsupervised Analysis.- Supervised Microarray Data Analysis.- Nearest Neighbors.- Support Vector Machines.- Methods To Improve Classifier Performance.- Genetic and Biochemical Networks.- Additional Methods For Microarray Data Analysis.- Microarray Data Visualization.- Microarray Data Standardization And Integration.- Microarray Gene Expression Markup Language (MAGE-ML).- Microarray Data Repositories.- Challenges In Microarray Gene Expression Data Analysis.- Conclusions.- References.

Reviews

From the reviews: The book offers a broad coverage of many of the most important aspects of microarray technology ... . Despite the wide field broached by this book, one of its chief successes lies with the fact that in each chapter the authors provide a clear review of every step of the technique, including both its potential and pitfalls. This allows all readers, especially beginners, to understand the development and impact of this important technique. ... Readers will find this beginner's guide book very informative ... . (Guillermina Lopez-Bendito, Journal of Chemical Neuroanatomy, Vol. 30, 2005)

From the reviews: <p> The book offers a broad coverage of many of the most important aspects of microarray technology a ] . Despite the wide field broached by this book, one of its chief successes lies with the fact that in each chapter the authors provide a clear review of every step of the technique, including both its potential and pitfalls. This allows all readers, especially beginners, to understand the development and impact of this important technique. a ] Readers will find this beginnera (TM)s guide book very informative a ] . (Guillermina Lopez-Bendito, Journal of Chemical Neuroanatomy, Vol. 30, 2005)

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions