Overview

Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.

Full Product Details

Author:   Anton Posch
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2008
Volume:   425
Dimensions:   Width: 15.50cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.545kg
ISBN:  

9781617378799

ISBN 10:   1617378798
Pages:   340
Publication Date:   09 December 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Application of Fluorescence Dye Saturation Labeling for Differential Proteome Analysis of 1,000 Microdissected Cells from Pancreatic Ductal Adenocarcinoma Precursor Lesions.- Albumin and Immunoglobulin Depletion of Human Plasma.- Multi-Component Immunoaffinity Subtraction and Reversed-Phase Chromatography of Human Serum.- Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies.- Proteomics of Cerebrospinal Fluid: Methods for Sample Processing.- Sample Preparation of Bronchoalveolar Lavage Fluid.- Preparation of Nasal Secretions for Proteome Analysis.- Preparation of Urine Samples for Proteomic Analysis.- Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis.- Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells.- Sample Preparation for Mass Spectrometry Analysis of Formalin-Fixed Paraffin-Embedded Tissue.- Metalloproteomics in the Molecular Study of Cell Physiology and Disease.- Protein Extraction from Green Plant Tissue.- The Terminator: A Device for High-Throughput Extraction of Plant Material.- Isolation of Mitochondria from Plant Cell Culture.- Isolation and Preparation of Chloroplasts from Arabidopsis thaliana Plants.- Isolation of Plant Cell Wall Proteins.- Isolation and Fractionation of the Endoplasmic Reticulum from Castor Bean (Ricinus communis) Endosperm for Proteomic Analyses.- Cell Wall Fractionation for Yeast and Fungal Proteomics.- Collection of Proteins Secreted from Yeast Protoplasts in Active Cell Wall Regeneration.- Sample Preparation Procedure for Cellular Fungi.- Isolation and Enrichment of Secreted Proteins from Filamentous Fungi.- Isolation and Solubilization of Cellular Membrane Proteins from Bacteria.- Isolation and Solubilization of Gram-Positive Bacterial Cell Wall-Associated Proteins.- Cell Fractionation of Parasitic Protozoa.

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