Overview

This volume is divided in six section covering the most experimental approaches involved in the study of the unfolded protein response (UPR) pathway. Chapters detail determination of unfolded protein levels, methods to study UPR signal transmission, analysing the outcomes of the UPR pathway activation, UPR studies in mammalian models, UPR in alternative models, and UPR and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, The Unfolded Protein Response: Methods and Protocols aims to describe key methods and approaches used in the study of the UPR pathway and its complex cellular implications. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Full Product Details

Author:   Roberto Pérez-Torrado
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2378
Weight:   0.845kg
ISBN:  

9781071617311

ISBN 10:   1071617311
Pages:   332
Publication Date:   06 January 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I Determination of unfolded protein levels 1 Measuring cysteine exposure in unfolded proteins with tetraphenylethene maleimide and its analogues Shouxiang Zhang and Yuning Hong   2 Fluorescence-based biosensors for the detection of the unfolded protein response Heinrich Kroukamp, Kai Peng, Ian T. Paulsen, and Riaan den Haan   3 Indirect methods to measure unfolded proteins in living cells using fluorescent proteins Elisabet Navarro-Tapia, and Roberto Pérez-Torrado   4 A platform technology for monitoring the Unfolded Protein Response Bhagyashree Bachhav, Carlos A. Origel Marmolejo, Yafet Arefeayne, and Laura Segatori   5 The use of fluorescent protein fusions to monitor the unfolded protein response and protein foldase-substrate interactions in plant protoplasts Rina Carrillo, Elizabeth Feldeverd, and David A. Christopher   Part II Methods to study UPR signal transmission   6 High-throughput analysis of protein turnover with tandem fluorescent protein timers Jia Jun Fung, Karla Blöcher-Juárez, and Anton Khmelinskii   7 Detection of HAC1 mRNA Splicing by RT-PCR in Saccharomyces cerevisiae Jagadeesh Kumar Uppala and Madhusudan Dey   Part III Analysing the outcomes of the UPR pathway activation   8 Determination of protein translation during the activation of the UPR Weihan Li and Robert H. Singer 9 Determination of the stability and intracellular (intra-nuclear) targeting and recruitment of pre-HAC1 mRNA in the Saccharomyces cerevisiae during the activation of UPR Sunirmal Paira and Biswadip Das   10 Assays to study IRE1 activation and signaling Paloma Moraga, Raul Aravena, Hery Urra, and Claudio Hetz   11 Analysis of XBP1 contribution to hyperosmolarity-induced lipid synthesis. Cecilia I. Casali, Leandro Parra, Luciana C. Erjavec, and María del Carmen Fernández Tome   12 Integrating an ER stress reporter for monitoring genome wide UPR-ER in budding yeast Shuvadeep Maity, Asmita Ghosh, and Kausik Chakraborty   Part IV UPR studies in mammalian models   13 Analyze mouse knockout models of UPR pathway elements Chao Zheng, Cheng Wang, Qiang Jie, and Liu Yang   14 In vitro stimulation of IRE1a/XBP1-deficient B cells with LPS Huabin Zhu, Chen Jiang, Randal J. Kaufman, Honglin Li, and Nagendra Singh   15 Detection of PERK signaling in the central nervous system Zhixin Lei, Sarrabeth Stone, and Wensheng Lin   Part V UPR in alternative models   16 Methods to study the mitochondrial unfolded protein response (UPRmt) in Caenorhabditis elegans Simon Haeussler and Barbara Conradt   17 Drosophila Unfolded Protein Response (UPR) assays in vitro and in vivo Hidetaka Kato, Deepika Vasudevan, and Hyung Don Ryoo   18 Protein preparation for proteomic analysis of the unfolded protein response in Arabidopsis thaliana Yunting Pu and Federica Brandizzi   Part VI UPR and disease   19 Structure-based drug discovery of IRE1 modulators Diana Pelizzari-Raymundo, Leif A. Eriksson, Eric Chevet, and Xavier Guillory   20 Chemical screening to detect ER stress regulators Mehrnoosh Ghafouri, Chester B. Gauss, and Andrew M. Fribley

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