Overview

Plant-Pathogen Interactions: Methods and Protocols provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts, which will help to develop better methods for disease control in plants and animals. Plant-Pathogen Interactions: Methods and Protocols reviews methods for engineering resistance to plant viruses, the utility of viral induced gene silencing and RNAi silencing, as well as advances in genomics and proteomics that have lead to new methods to identify genes and proteins. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls.

Full Product Details

Author:   Pamela C. Ronald
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2007
Volume:   354
Dimensions:   Width: 15.20cm , Height: 1.30cm , Length: 22.90cm
Weight:   0.411kg
ISBN:  

9781617375767

ISBN 10:   1617375764
Pages:   248
Publication Date:   09 December 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

The Use of Protoplasts to Study Innate Immune Responses.- Marker-Exchange Mutagenesis and Complementation Strategies for the Gram-Negative Bacteria Xanthomonas oryzae pv. oryzae.- Whole-Genome Analysis to Identify Type III-Secreted Effectors.- In planta Expression of Oomycete and Fungal Genes.- Use of Nipponbare BAC Clones for Physical Mapping of an R Gene Locus in Rice.- Agrobacterium-Mediated Transformation to Create an Insertion Library in Magnaporthe grisea.- Identification of Components in Disease-Resistance Signaling in Arabidopsis by Map-Based Cloning.- Yeast Two-Hybrid Approaches to Dissecting the Plant Defense Response.- Use of Rolling-Circle Amplification for Large-Scale Yeast Two-Hybrid Analyses.- Preparative Denaturing Isoelectric Focusing for Enhancing Sensitivity of Proteomic Studies.- Use of Massively Parallel Signature Sequencing to Study Genes Expressed During the Plant Defense Response.- Use of Microarray Analysis to Dissect the Plant Defense Response.- Use of Robust-Long Serial Analysis of Gene Expression to Identify Novel Fungal and Plant Genes Involved in Host-Pathogen Interactions.- Analysis of Gene Function in Rice Through Virus-Induced Gene Silencing.- Use of RNA Interference to Dissect Defense-Signaling Pathways in Rice.- Virus-Induced Gene Silencing in Plant Roots.- Methods for Engineering Resistance to Plant Viruses.- Development of Genetically Engineered Resistant Papaya for papaya ringspot virus in a Timely Manner.

Reviews

From the reviews: In a highly lucid manner, this book describes new developments in generic techniques and others which are exclusive to plant pathologists. As a result this book will be of interest to seasoned practioners in the field as much as new post-graduate students. ... The presentation of each section is exemplary, with a useful summary, a concise introduction to each section and a step-by-step protocol. ... this is a wide-ranging protocols book that will be useful to plant pathologists at all stages in their careers. (Dr Luis Mur, Molecular Biotechnology, 2007) The book is divided into 18 chapters ... . this book is an excellent reference for people interested in validating or improving existing lab protocols. ... serve as a basic reference work for those entering into a new research area. ... Texts are clearly written and the tabular format makes it easier to adopt novel methods step-by-step. This makes the book also a valuable tool for people involved in teaching. All in all, I can recommend this book as a good value for the money. (Ulrich Schaffrath, Journal of Plant Physiology, Vol. 165, 2008) In this volume, the editor has assembled a versatile collection of papers that focus on large-scale analyses of plant-pathogen interactions. ... With contributions from over 40 experts in their fields, this stepwise set of protocols ... will certainly be of interest to molecular plant-pathogen researchers. (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, 2007)

From the reviews: In a highly lucid manner, this book describes new developments in generic techniques and others which are exclusive to plant pathologists. As a result this book will be of interest to seasoned practioners in the field as much as new post-graduate students. ! The presentation of each section is exemplary, with a useful summary, a concise introduction to each section and a step-by-step protocol. ! this is a wide-ranging protocols book that will be useful to plant pathologists at all stages in their careers. (Dr Luis Mur, Molecular Biotechnology, 2007) The book is divided into 18 chapters ! . this book is an excellent reference for people interested in validating or improving existing lab protocols. ! serve as a basic reference work for those entering into a new research area. ! Texts are clearly written and the tabular format makes it easier to adopt novel methods step-by-step. This makes the book also a valuable tool for people involved in teaching. All in all, I can recommend this book as a good value for the money. (Ulrich Schaffrath, Journal of Plant Physiology, Vol. 165, 2008) In this volume, the editor has assembled a versatile collection of papers that focus on large-scale analyses of plant-pathogen interactions. ! With contributions from over 40 experts in their fields, this stepwise set of protocols ! will certainly be of interest to molecular plant-pathogen researchers. (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, 2007)

From the reviews: In a highly lucid manner, this book describes new developments in generic techniques and others which are exclusive to plant pathologists. As a result this book will be of interest to seasoned practioners in the field as much as new post-graduate students. ... The presentation of each section is exemplary, with a useful summary, a concise introduction to each section and a step-by-step protocol. ... this is a wide-ranging protocols book that will be useful to plant pathologists at all stages in their careers. (Dr Luis Mur, Molecular Biotechnology, 2007) The book is divided into 18 chapters ... . this book is an excellent reference for people interested in validating or improving existing lab protocols. ... serve as a basic reference work for those entering into a new research area. ... Texts are clearly written and the tabular format makes it easier to adopt novel methods step-by-step. This makes the book also a valuable tool for people involved in teaching. All in all, I can recommend this book as a good value for the money. (Ulrich Schaffrath, Journal of Plant Physiology, Vol. 165, 2008) In this volume, the editor has assembled a versatile collection of papers that focus on large-scale analyses of plant-pathogen interactions. ... With contributions from over 40 experts in their fields, this stepwise set of protocols ... will certainly be of interest to molecular plant-pathogen researchers. (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, 2007)

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