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OverviewPlant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition. Full Product DetailsAuthor: S.B. Gelvin , R.A. SchilperoortPublisher: Springer Imprint: Springer Edition: 2nd ed. 2000. Softcover reprint of the original 2nd ed. 2000 Dimensions: Width: 15.50cm , Height: 1.00cm , Length: 23.50cm Weight: 0.317kg ISBN: 9789401176552ISBN 10: 9401176558 Pages: 161 Publication Date: 25 December 2012 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsE3: Yeast as versatile tool in transcription factor research.- H5: The construction of bacterial artificial chromosome (BAC) libraries.- N1: Gene transfer mediated by site-specific recombination systems.- N2: Use of the R-RS site-specific recombination system in plants.- N3: Cre-lox directed integration of transgenes into the tobacco genome.- 01: Plant actin isolation and characterization.ReviewsAbout the first edition: ' Altogether, Plant Molecular Biology Manual is a very useful guide for the practical application of modern methods of molecular biology in plant sciences and is therefore highly recommended. ' Plant Breeding ' ...every researcher in plant molecular biology should take a look at this book ' New Phytologist About the first edition: ` Altogether, Plant Molecular Biology Manual is a very useful guide for the practical application of modern methods of molecular biology in plant sciences and is therefore highly recommended. ' Plant Breeding ` ...every researcher in plant molecular biology should take a look at this book ' New Phytologist Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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