Overview

In the late-90s, antisense methodology has moved from in vitro studies to in vivo studies and first human trials. While the basic concept of antisense technology is simple, the methodological problems associated with its use are numerous and complex. Antisense-based methods have proven to be a field of research where careful attention to experimental protocols and appropriate controls is necessary. This book emphasizes the application of antisense oligonucleotides, and is a guide for the identification of antisense and non-antisense effects in different experimental settings. The work is organized into three sections, antisense application in vitro, antisense application in vivo (animal models) and finally, clinical antisense studies. Where at all possible, the methods are described in sufficient detail to allow reproduction of a given experiment. It should be of interest to researchers in immunology, cancer research, pharmacology and internal medicine; and physicians conducting clinical studies in these fields.

Full Product Details

Author:   Gunther Hartmann ,  Stefan Endres
Publisher:   Springer
Imprint:   Springer
Edition:   1999 ed.
Volume:   4
Dimensions:   Width: 15.50cm , Height: 1.40cm , Length: 23.50cm
Weight:   1.090kg
ISBN:  

9780792385394

ISBN 10:   079238539
Pages:   205
Publication Date:   31 May 1999
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

I: The chemistry of antisense oligonucleotides.- 1. Chemical synthesis and purification of phosphorothioate antisense oligonucleotides.- 2. How to choose optimal antisense targets in an mRNA.- II: Antisense application in vitro.- 3. How to characterize and improve oligonucleotide uptake into leukocytes.- 4. Strategies for targeted uptake of antisense oligonucleotides in hepatocytes.- 5. How to exclude immunostimmulatory and other nonantisense effects of antisense oligonucleotides.- 6. Testing antisense oligonucleotides in controlled cell culture assays.- 7. Inhibition of TNF synthesis by antisense oligonucleotides.- 8. Inhibition of IGF-1 receptor expression by antisense oligonucleotides in vitro and in vivo.- III: Antisense application in vivo.- 9. How to test antisense oligonucleotides in animals.- 10. Lipid-based carriers for the systemic delivery of antisense drugs.- 11. Designing a clinical antisense study.

Reviews

Author Information

Gunther Hartmann, M.D. is a clinical and research fellow at the Division of Clinical Pharmacology, University of Munich. Stefan Endres, M.D. is an internist with subspecialties in gastroenterology and intensive care medicine. He is Chief of the Division of Clinical Pharmacology of the medical faculty at the University of Munich.

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions