Overview

Bacterial Efflux Systems, Volume 722 covers experimental and computational approaches to study the function, structure, and mechanism of bacterial efflux pump systems. The methods covered include preparation of samples for structural and functional analyses and in vivo activity analysis. Specific chapters in this release cover Efflux pump functional assays, NMR and functional analysis efflux pumps, Methods for saturation mutagenesis and functional characterization, Cooperation and functional interplay between pumps, Mapping interactions, HDX-MS, and much more. Other sections cover Recombinant Expression and purification, Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach, Reconstitution of rnd and mfs efflux pumps for structural biology, CusCBA system, Expression and purification of efflux pumps of Gram positives, Expression and purification of efflux pumps of archaea, ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc, In situ structural analysis of efflux assemblies by cryoET, Molecular dynamics simulations, and so much more.

Full Product Details

Author:   Ben Luisi (Department of Biochemistry, University of Cambridge, U.K.) ,  Ben Luisi (Department of Biochemistry, University of Cambridge, U.K.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Weight:   0.450kg
ISBN:  

9780443429590

ISBN 10:   0443429596
Pages:   564
Publication Date:   24 November 2025
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Efflux pump functional assays 2. NMR and functional analysis efflux pumps 3. Methods for saturation mutagenesis and functional characterisation 4. Cooperation and functional interplay between pumps 5. Mapping interactions, HDX-MS Section II: Recombinant Expression and purification 6. Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach 7. Reconstitution of rnd and mfs efflux pumps for structural biology 8. CusCBA system 9. Expression and purification of efflux pumps of Gram positives 10. Expression and purification of efflux pumps of archaea 11. ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc Section III. Structural and computational studies 12. In situ structural analysis of efflux assemblies by cryoET 13. Molecular dynamics simulations 14. CryoEM analysis of efflux pumps 15. Crystallographic studies of transporters with transport substrates and inhibitors Section IV. In vivo expression and activity and physiological roles 16. Assembly and biogenesis 17. Heterogeneity of efflux pump expression in populations

Reviews

Author Information

Ben Luisi works in the Department of Biochemistry at the University of Cambridge, where he has an appointment as professor of structural biology. He and his colleagues study the structure and mechanism of efflux pumps and the riboregulatory machinery that help to control the expression of the pumps and other complex systems in bacteria.

Tab Content 6

Author Website:  

Countries Available

All regions