Overview

A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses-the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer.

Full Product Details

Author:   Stuart N. Isaacs
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2004
Volume:   269
Dimensions:   Width: 15.20cm , Height: 2.10cm , Length: 22.90cm
Weight:   0.606kg
ISBN:  

9781617374371

ISBN 10:   1617374377
Pages:   396
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Working Safely with Vaccinia Virus.- Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers.- Construction of Recombinant Vaccinia Virus.- Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses.- Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA.- Construction of cDNA Libraries in Vaccinia Virus.- Construction and Isolation of Recombinant MVA.- Growing Poxviruses and Determining Virus Titer.- Rapid Preparation of Vaccinia Virus DNA Template for Analysis and Cloning by PCR.- Orthopoxvirus Diagnostics.- An In Vitro Transcription System for Studying Vaccinia Virus Early Genes.- An In Vitro Transcription System for Studying Vaccinia Virus Late Genes.- Studying Vaccinia Virus RNA Processing In Vitro.- Methods for Analysis of Poxvirus DNA Replication.- Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia Virus.- Pox, Dyes, and Videotape.- Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance.- Monitoring of Human Immunological Responses to Vaccinia Virus.- Vaccinia Virus as a Tool for Immunologic Studies.- Mouse Models for Studying Orthopoxvirus Respiratory Infections.- Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors.- Use of Dual Recombinant Vaccinia Virus Vectors to Assay Viral Glycoprotein-Mediated Fusion with Transfection-Resistant Primary Cell Targets.- Poxvirus Bioinformatics.- Preparation and Use of Molluscum Contagiosum Virus from Human Tissue Biopsy Specimens.

Reviews

From the foreword... The right book at the right time. -Bernard Moss ...a collection of up-to-date protocols...the book is intended for virologists devoted to poxvirus research and scientists in vaccine research and some biotechnology area. - Chemotherapy

From the foreword... The right book at the right time. -Bernard Moss ...a collection of up-to-date protocols...the book is intended for virologists devoted to poxvirus research and scientists in vaccine research and some biotechnology area. - Chemotherapy

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions