Free Delivery Over $100
|
|
|||
|
||||
OverviewAgrobacterium tumefaciens causes crown gall disease on plants and is the primary vector in genetic engineering of plants. Agrobacterium transports DNA and proteins to the plant cell via a Type IV Secretion System. This system is similar to secretion systems in human pathogens and those used for DNA exchange during bacterial conjugation. To better understand this process, a genetic screen using yeast as the genetically engineered host was developed to identify host proteins involved in transformation. While developing this screen, factors that alter transformation of yeast were identified. The yeast two-hybrid (Y2H) assay was used to identify protein interactions between the exported proteins and the secretion system. Additional studies indicate the secreted proteins are targeted to the secretion system via a conserved transport sequence. The Y2H system was also used to assay for interactions between proteins of the secretion system. Together, these studies provide insights into transporter complex protein subassemblies, the process of protein export during Agrobacterium transformation, and contribute to understanding homologous systems in human pathogens and bacterial conjugation. Full Product DetailsAuthor: Cheryl HackworthPublisher: VDM Verlag Dr. Muller Aktiengesellschaft & Co. KG Imprint: VDM Verlag Dr. Muller Aktiengesellschaft & Co. KG Dimensions: Width: 15.20cm , Height: 0.80cm , Length: 22.90cm Weight: 0.233kg ISBN: 9783639137453ISBN 10: 3639137450 Pages: 152 Publication Date: 03 April 2009 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
||||
