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OverviewShA multimetal resistant strain of Enterobacter cloacae (E. cloacae) grew in the presence of 40 mM selenite and demonstrated the ability to transform it into a red precipitate. Established growth curve studies in M-9 minimal salts medium showed that the E. cloacae strain grew in the presence of selenite only when the medium was supplemented with L-cysteine. During early log-phase, selenite or water was added. One hour later, cells were harvested for protein analysis by two dimensional gel electrophoresis (2DGE). Protein profiles of cultures grown under the resistant (cysteine) and sensitive (no cysteine) conditions were compared and differentially expressed polypeptides spots were excised and sent to Ohio State University for identification by mass spectrometry. The identified proteins were classified under three possible functions: 1) oxidative stress response, 2) selenium processing and 3) selenite efflux. None of the identified proteins indicated a possible role for cysteine in selenite resistance.orttitle Full Product DetailsAuthor: Nathaniel BarasaPublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.60cm , Length: 22.90cm Weight: 0.150kg ISBN: 9783843352604ISBN 10: 3843352607 Pages: 96 Publication Date: 22 November 2011 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In stock We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |