Proteomic Characterization of Selenite Resistance

Author:   Nathaniel Barasa
Publisher:   LAP Lambert Academic Publishing
ISBN:  

9783843352604


Pages:   96
Publication Date:   22 November 2011
Format:   Paperback
Availability:   In stock   Availability explained
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Proteomic Characterization of Selenite Resistance


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Overview

ShA multimetal resistant strain of Enterobacter cloacae (E. cloacae) grew in the presence of 40 mM selenite and demonstrated the ability to transform it into a red precipitate. Established growth curve studies in M-9 minimal salts medium showed that the E. cloacae strain grew in the presence of selenite only when the medium was supplemented with L-cysteine. During early log-phase, selenite or water was added. One hour later, cells were harvested for protein analysis by two dimensional gel electrophoresis (2DGE). Protein profiles of cultures grown under the resistant (cysteine) and sensitive (no cysteine) conditions were compared and differentially expressed polypeptides spots were excised and sent to Ohio State University for identification by mass spectrometry. The identified proteins were classified under three possible functions: 1) oxidative stress response, 2) selenium processing and 3) selenite efflux. None of the identified proteins indicated a possible role for cysteine in selenite resistance.orttitle

Full Product Details

Author:   Nathaniel Barasa
Publisher:   LAP Lambert Academic Publishing
Imprint:   LAP Lambert Academic Publishing
Dimensions:   Width: 15.20cm , Height: 0.60cm , Length: 22.90cm
Weight:   0.150kg
ISBN:  

9783843352604


ISBN 10:   3843352607
Pages:   96
Publication Date:   22 November 2011
Audience:   General/trade ,  General
Format:   Paperback
Publisher's Status:   Active
Availability:   In stock   Availability explained
We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately.

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