Production and Purification of Laccase from White rot fungi

Author:   Priyanka Ghantasala
Publisher:   LAP Lambert Academic Publishing
ISBN:  

9783659779244


Pages:   140
Publication Date:   05 October 2015
Format:   Paperback
Availability:   In stock   Availability explained
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Production and Purification of Laccase from White rot fungi


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Overview

White rot fungi are unique in their strong ability to extensively degrade xenobiotics and recalcitrant pollutants have been widely applied in bioremediation and environmental biotechnology. These secrete extracellular enzyme laccase which plays an important role in degradation of xenobiotics. In the present study the white rot basidiomycete Trametes versicolor NCIM 1086 was tested for its ability to produce laccase on natural agricultural residues. Palm fibre was selected as substrate due to its high peak enzyme activity, the maximum laccase activity was obtained in preliminary studies by incubating flasks containing palm fibre of 80% initial moisture content (pH 4.0) as substrate supplemented with mannitol (1.0% w/v), peptone(1% w/v), CuSO4 (1mM) and 4 ml of T.versicolor conidial suspension for 7days at 300C. Inoculum volume, initial moisture content, carbon supplement concentration and CuSO4 concentration were selected for optimization by Central Composite Design (CCD) which showed significant effect on laccase production in the preliminary studies. Decolorization studies were carried out on textile effluent dyes by partially purified laccase.

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Author:   Priyanka Ghantasala
Publisher:   LAP Lambert Academic Publishing
Imprint:   LAP Lambert Academic Publishing
Dimensions:   Width: 15.20cm , Height: 0.80cm , Length: 22.90cm
Weight:   0.213kg
ISBN:  

9783659779244


ISBN 10:   3659779245
Pages:   140
Publication Date:   05 October 2015
Audience:   General/trade ,  General
Format:   Paperback
Publisher's Status:   Active
Availability:   In stock   Availability explained
We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately.

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