Overview

This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.

Full Product Details

Author:   Roberto Botelho
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2017
Volume:   1519
Weight:   6.975kg
ISBN:  

9781493982417

ISBN 10:   1493982419
Pages:   360
Publication Date:   08 June 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Phagocytosis: Hungry, Hungry Cells.- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry.- Quantitative Efferocytosis Assays.- Quantifying Phagocytosis by Immunofluorescence and Microscopy.- Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage and Cell Death Following Exposure of Macrophages to Silica Particles.- Quantitative Live-cell Fluorescence Microscopy during Phagocytosis.- Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers.- Quantitative Immunofluorescence to Study Phagosome Maturation.- Using Fluorescence Resonance Energy Transfer-Based Biosensors to Probe Rho Gtpase Activation during Phagocytosis.- Analysis of LC3-associated Phagocytosis and Antigen Presentation.- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells.- Measuring Phagosomal pH by Fluorescence Microscopy.- Image-Based Analysis of Phagocytosis – Measuring Engulfment and Internalization.- Fluorometric Approaches to Measuring Reductive and Oxidative Events in Phagosomes.- Simultaneous Analysis of Multiple Lumenal Parameters of Individual Phagosomes Using High-Content Imaging.- Salmonella Enterica Internalization and Vacuole Lysis in Epithelial Cells.- Bacterial Binding, Phagocytosis and Killing: Measurements Using Colony Forming Units.- Filamentous Bacteria as Targets to Study Phagocytosis.- Growing and Handling of Mycobacterium Tuberculosis for Macrophage Infection Assays.- Mycobacterium Tuberculosis: Readouts of Bacterial Fitness and The Environment within the Phagosome.- Using Flow Cytometry to Analyse Cryptococcus Infection in Macrophages.>

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