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OverviewHands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. Full Product DetailsAuthor: John HowlPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 2005 ed. Volume: 298 Dimensions: Width: 15.50cm , Height: 1.70cm , Length: 23.50cm Weight: 0.570kg ISBN: 9781588293176ISBN 10: 1588293173 Pages: 262 Publication Date: 28 February 2005 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsCommon Strategies.- Fundamentals of Modern Peptide Synthesis.- Chimerism.- Synthetic Methodologies and Applications.- Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars.- Synthesis of Linear, Branched, and Cyclic Peptide Chimera.- Synthesis of Cell-Penetrating Peptides for Cargo Delivery.- Incorporation of Phosphotyrosyl Mimetic 4- (Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides.- Expressed Protein Ligation for Protein Semisynthesis and Engineering.- Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides.- Quenched Fluorescent Substrate- Based Peptidase Assays.- A Convenient Method for Synthesis of Cyclic Peptide Libraries.- High-Throughput Peptide Synthesis.- Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C - Terminal Modified Peptides.- 13 Synthesis of Peptide Bioconjugates.- Practical Guides.- Protein Identification by Mass Spectrometric Analyses of Peptides.- Manual Solid Phase Synthesis of Glutathione Analogs.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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