Overview

A panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of the endothelin system from its synthesis and metabolism, to its function in health and disease. Theses methods use state-of-the-art molecular techniques to quantify the expression of mRNA for both endothelin receptors and the endothelin converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.

Full Product Details

Author:   Janet J. Maguire ,  Anthony P. Davenport
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2002
Volume:   206
Dimensions:   Width: 15.20cm , Height: 1.40cm , Length: 22.90cm
Weight:   0.415kg
ISBN:  

9781617372919

ISBN 10:   1617372919
Pages:   242
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Endothelin Peptide Protocols.- Immunocytochemical Localization of Endothelin Peptides, Precursors, and Endothelin-Converting Enzymes.- Analysis of Endothelins by Enzyme-Linked Immunosorbent Assay and Radioimmunoassay.- Separation of Endothelin Isoforms by High Performance Liquid Chromatography and Detection by Radioimmunoassay.- Endothelin Receptor Protocols.- Radioligand binding assays and quantitative autoradiography of endothelin receptors.- Using Receptor Antagonists in Binding Studies to Characterize a Mammalian Endothelin Receptor.- Quantification of Endothelin Receptor mRNA by Competitive RT-PCR.- Endothelin-Converting Enzyme Protocols.- Detection and Assessment of Endothelin-Converting Enzyme Activity.- Quantitative Measurement of mRNA Levels by RT-PCR.- Evaluation of Endothelin-Converting Enzyme Inhibitors Using Cultured Cells.- Endothelin-Converting Enzyme Activity in Vascular Smooth Muscle Preparations In Vitro.- In Vitro Functional Assays.- Characterization of a New Endothelin Receptor Ligand by In Vitro Assays.- Use of In Vitro Organ Cultures of Human Saphenous Vein as a Model for Intimal Proliferation.- In Vivo Functional Assays.- Investigation of the Endothelin System in Experimental Heart Failure.- Local Forearm Vasoconstriction to Endothelin-1 Measured by Venous Occlusion Plethysmography In Vivo.

Reviews

This book is excellent for what it intends to be: to provide a coherent series of protocols on a focused topic. It efficiently integrates biochemical, pharmacological, physiological and molecular biological methods... -Acta Cardiologica

""This book is excellent for what it intends to be: to provide a coherent series of protocols on a focused topic. It efficiently integrates biochemical, pharmacological, physiological and molecular biological methods..."" -Acta Cardiologica

This book is excellent for what it intends to be: to provide a coherent series of protocols on a focused topic. It efficiently integrates biochemical, pharmacological, physiological and molecular biological methods... -Acta Cardiologica

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