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OverviewA collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. Full Product DetailsAuthor: Piet HerdewijnPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of hardcover 1st ed. 2005 Volume: 288 Dimensions: Width: 15.20cm , Height: 2.50cm , Length: 22.90cm Weight: 0.660kg ISBN: 9781617374418ISBN 10: 1617374415 Pages: 436 Publication Date: 10 November 2010 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsSynthesis of DNA Using a New Two-Step Cycle.- RNA Synthesis Using 2?-O-(Tert-Butyldimethylsilyl) Protection.- RNA Oligonucleotide Synthesis Via 5?-Silyl-2?-Orthoester Chemistry.- Dimethylthiarum Disulfide.- Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes.- Di- and Oligonucleotide Synthesis Using H-Phosphonate Chemistry.- Solid-Phase Synthesis of Circular Oligonucleotides.- Locked Nucleic Acid Synthesis.- Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-Oligomers.- Base-Modified Oligonucleotides With Increased Duplex Stability.- of Hypermodified Nucleotides in RNA.- Chemical Methods for Peptide-Oligonucleotide Conjugate Synthesis.- Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms.- Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips.- Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides.- Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis.- Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays.- High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing.- Nonenzymatic Template-Directed RNA Synthesis.- DNase I Footprinting of Small Molecule Binding Sites on DNA.- Gene Targeting Using Peptide Nucleic Acid.- Nucleic Acid Library Construction Using Synthetic DNA Constructs.- In Vitro Selection From Combinatorial Nucleic Acid Libraries.- In Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and Proteins.- Short Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference Mechanism.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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