Overview

Next Generation Sequencing and Microbial Ecology is a very timely and succinct compilation of the current developments in nucleic acid sequencing methods and their applications especially in helping understand microbial interaction and their form, community structure and function in various biotic communities. The book introduces the readers to different methods of sequencing DNA - from conventional to next generation sequencing, and discussing invariably the limitation and advantages of each of these methods, with respect to microbial ecology studies. The book highlights several gene families which have been favourably taken up in the recent past for the purpose of dissecting the communities and their possible roles. In this book, we also discuss some very novel aspects of microbial ecology, like “the metagenomics approach”, which scientist have been able to work out mainly by culture independent approaches. We will highlight how this is helping us understand the structural and functional aspect i.e., “who is out there” and “what they are doing” in a number of different environments and the ecological significance of these aspects. We hope that the book will be useful for students, researchers and teachers as a reference for choosing an appropriate sequencing approach, with respect to the gene family and analytical methods for studying microbes and their role in a community/ecosystem. Suggestions for improving the book are cordially invited.

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9781773611945

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Dr Prashant Pant, is an Assistant Professor of Botany in Dyal Singh College, University of Delhi, New Delhi, India. He has been teaching for more than 5 years now and his main areas have been Bioinformatics, Molecular Biology and Genomics at the college. His PhD was on understanding the Microbial Succession using a Metagenomic approach in a Barren mined-out site undergoing ecological restoration. For this, he deployed many molecular and computational Biology tool and techniques. He has several publications (articles and book chapters) in International Journals and International Publications. Currently, he is also Chief Editor of Bioinformatics Review and a co-founder/Executive Chief Editor of a Science Journal in Hindi Language Swadeshi Vigyan for the native language speaking communities. Both the Journals are a service to the scientific community as they are fully open access. Dr Pratibha Pant, is an Assistant Professor of Botany in Daulat Ram College, University of Delhi, New Delhi, India and has a teaching experience of more than 6 years. She is a plant virologist and biotechnologist by training and has done her PhD in Characterization of Begomoviral genomes from Papaya from Department of Botany, University of Delhi. During her PhD, she utilized a combination of molecular biology and Computational Biology tools to analyze begomoviral genomes and to her credit, a couple of novel begomoviruses have been isolated, sequenced and reported in public databases. Dr Prerna Pandey, a biotechnologist with several years of wet lab research experience, worked at International Center for Genetic Engineering and Biotechnology, New Delhi. Her field of research in PhD included isolation and molecular characterization of geminiviruses, genome sequencing, gene annotation, and gene silencing using the RNA interference technology. She has also worked at Transasia Biomedicals and Advance Enzyme Technologies as a scientist. Prerna has published papers in peer-reviewed journals, and has submitted a number of annotated Geminiviral genome sequences in the GenBank, including two novel ones. She has also completed her editing and proof-reading courses from Society for the promotion of Editors and Proof-readers, UK and now works as a freelance scientific editor and writer. When Prerna is not busy with her assignments, she loves to explore historical places.

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