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OverviewHigh Quality Content by WIKIPEDIA articles! Nucleic Acid Sequence Based Amplification (NASBA) is a method in molecular biology which is used to amplify RNA sequences. NASBA was developed by J Compton in 1991, who defined it as a primer-dependent technology that can be used for the continuous amplification of nucleic acids in a single mixture at one temperature. Immediately after the invention of NASBA it was used for the rapid diagnosis and quantification of HIV-1 in patient sera. Although RNA can also be amplified by PCR using a reverse transcriptase (in order to synthesize a complementary DNA strand as a template), NASBA's main advantage is that it works at isothermic conditions - usually at a constant temperature of 41C. NASBA has been introduced into medical diagnostics, where it has been shown to give quicker results than PCR, and it can also be more sensitive. Full Product DetailsAuthor: Lambert M. Surhone , Miriam T. Timpledon , Susan F. MarsekenPublisher: VDM Publishing House Imprint: VDM Publishing House Dimensions: Width: 22.90cm , Height: 0.50cm , Length: 15.20cm Weight: 0.142kg ISBN: 9786130542047ISBN 10: 6130542046 Pages: 88 Publication Date: 23 June 2010 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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