Overview

This is a collection of cutting-edge mycoplasma methods for the detection, isolation, identification, characterization, and genetic manipulation of the pathogenic mycoplasmas. These step-by-step methods are crafted for successful reproducibility and include biochemical, genetic, and molecular techniques essential to understanding pathogenecity and adhesion to host cells. They also cover the detection of mycoplasmas in cell cultures, an important tool not only in viral diagnosis and research, but also in the production of vaccines and various biological products.

Full Product Details

Author:   Roger J. Miles ,  Robin A. J. Nicholas
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 1998
Volume:   104
Dimensions:   Width: 15.20cm , Height: 1.90cm , Length: 22.90cm
Weight:   0.512kg
ISBN:  

9781617370618

ISBN 10:   1617370614
Pages:   330
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Medical Significance of Mycoplasmas.- The Veterinary Significance of Mycoplasmas.- Recovery of Human Mycoplasmas.- Recovery of Mycoplasmas from Animals.- Recovery of Mycoplasmas from Birds.- Cultivation of Ureaplasmas.- Quality Control Testing of Mycoplasma Medium.- Biochemical Characteristics in Mycoplasma Identification.- Enzyme Analysis.- Determination of Substrate Utilization Rates by Mycoplasmas.- Serological Identification of Mycoplasmas by Growth and Metabolism Inhibition Tests.- Identification of Mycoplasmas by Dot Immunobinding on Membrane Filtration (MF Dot).- Identification of Mycoplasmas by Immunofluorescence.- Diagnostic Application of Monoclonal Antibody (MAb)-Based Sandwich ELISAs.- Immunohistochemical Staining of Fixed Tissues.- Extraction of DNA from Mycoplasmas.- Characterization of Mycoplasmas by PCR and Sequence Analysis with Universal 16S rDNA Primers.- PCR and RFLP Methods for the Specific Detection and Identification of Mycoplasma mycoides subsp. mycoides SC.- Characterization of Mycoplasmas by RAPD Fingerprinting.- Classification of Isolates by DNA-DNA Hybridization.- Insertion Sequence Analysis.- Detection of Mycoplasmas in Cell Cultures by Cultural Methods.- Detection of Mycoplasmas in Cell Culture by Fluorescence Methods.- Transformation of Mycoplasmas.- Transposon Mutagenesis.- Demonstration of Extrachromosomal Elements.- Expression of Foreign Genes in Acholeplasma laidlawii.- Mycoplasma Gene Expression in Escherichia coli.- Polyacrylamide Gel-Electrophoresis Separation of Whole-Cell Proteins.- Immunological and Biochemical Characterization of Membrane Proteins.- Detection and Analysis of Mycoplasma Adhesins.- Transmission Electron Microscopy and Immunogold Staining of Mollicute Surface Antigens.

Reviews

The value of this book lies in the amount of technical detail provided. . .this should enable the interested investigator to carry out the protocols described without recourse to other publications. -Journal of Medical Microbiology

The value of this book lies in the amount of technical detail provided...this should enable the interested investigator to carry out the protocols described without recourse to other publications. -Journal of Medical Microbiology

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