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Author:   Gerd Pluschke ,  Katharina Röltgen
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2387
Weight:   0.470kg
ISBN:  

9781071617816

ISBN 10:   1071617818
Pages:   232
Publication Date:   14 October 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Overview: Mycobacterium ulcerans Disease (Buruli ulcer).- Immunohistochemistry: A Method to Analyze M.ulcerans Infected Skin Tissue.- Primary Isolation of Mycobacterium ulcerans.- Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance.- Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture.- DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR.- Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans.- Detection of Mycobacterium ulcerans DNA using Real-Time PCR.- Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR.- Methods and Approaches for Buruli ulcer Surveillance in Africa: Lessons Learnt and Future Directions.- Overview: Mycolactone, The Macrolide Toxin of Mycobacterium ulcerans.- Conditionsfor Handling and Optimal Storage of Mycolactone.- Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples.- Detection of Mycolactone by Thin Layer Chromatography.- Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone.- Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61.- Overview: Development of Drugs against Mycobacterium ulcerans.- In Vitro Assessment of Drug Activity against Mycobacterium ulcerans.- Drug Efficacy Testing in the Mouse Footpad Model of Buruli ulcer.- Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans.- Determination of Bioenergetic Parameters in Mycobacterium ulcerans.

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