Overview

This fully updated edition explores the latest techniques to study the challenging, and at times dangerous, genus of bacteria known as mycobacteria with basic methods that are still required for mycobacteriology along with the newer or improved methods that have been developed. The volume features chapters on the basics of DNA isolation, protein isolation, and lipid isolation, as well as more sophisticated techniques for isolation of ribosomes, and continues with sections involving analyzing subcellular fractions, culture methods, sequencing technology, in vitro models, molecular methods, as well as drug discovery applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Mycobacteria Protocols, Fourth Edition serves as an ideal guide for those starting out with their mycobacteria research and also for those who have worked with it for decades.

Full Product Details

Author:   Tanya Parish ,  Anuradha Kumar
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   4th ed. 2021
Volume:   2314
Weight:   1.407kg
ISBN:  

9781071614624

ISBN 10:   1071614622
Pages:   736
Publication Date:   08 July 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Culturing Mycobacteria             Elizabeth Wallace, Debra Hendrickson, Nicholas Tolli, Carolina Mehaffy, María Peña, Jerry A. Nick, Phillip Knabenbaur, Jackson Watkins, Anne Simpson, Anita G. Amin, Delphi Chatterjee, Karen M. Dobos, Ramanuj Lahiri, Linda Adams, Michael Strong; Max Salfinger, Rebecca Bradford, Timothy T. Stedman, Marco A. Riojas, and Manzour Hernando Hazbón   2. DNA Isolation from Mycobacteria             Heena Jagatia and Daire Cantillon   3. Extraction and Separation of Mycobacterial Proteins             Megan Lucas, Joan M. Ryan, Jackson Watkins, Kala Early, Nicole A. Kruh-Garcia, Carolina Mehaffy, and Karen M. Dobos   4. Lipid and Lipoarabinomannan Isolation and Characterization             Marie-Antoinette Lanéelle, Lucie Spina, Jérôme Nigou, Anne Lemassu, and Mamadou Daffé   5. Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria             Yunlong Li, Pooja Keshavan, Jamie H. Corro, Ravi K. Koripella, Rajendra K. Agrawal, and Anil K. Ojha   6. Macrophage Infection Models for Mycobacterium tuberculosis             Benjamin K. Johnson, Sean M. Thomas, Andrew J. Olive, and Robert B. Abramovitch   7. Novel Single-Cell and High Throughput Microscopy Techniques to Monitor Dictyostelium discoideum-Mycobacterium marinum Infection Dynamics             Manon Mottet, Cristina Bosmani, Nabil Hanna, Jahn Nitschke, Louise Lefrancois, and Thierry Soldati   8. Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy             Giulia Manina and Neeraj Dhar   9. Measuring Efflux and Permeability in Mycobacteria             Liliana Rodrigues, José A. Aínsa, and Miguel Viveiros   10. Drug Susceptibility Screening Using In Vitro Models of Hypoxic Non-Replicating Persistent Mycobacteria             Savannah E.R. Gibson, James Harrison, and Jonathan A.G. Cox   11. Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells             Sydney L. Solomon and Bryan D. Bryson   12. Electroporation of Mycobacteria             Tanya Parish   13. Gene Switching and Essentiality Testing             Amanda Claire Brown   14. Oligo-Mediated Recombineering and Its Use for Making SNPs, Knockouts, Insertions, and Fusions in Mycobacterium tuberculosis             Kenan C. Murphy   15. Using Proteolytic Hypomorphs to Detect Small Molecule Mechanism of Action             Eachan O. Johnson and Deborah T. Hung   16. CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria             Andrew I. Wong and Jeremy M. Rock   17. Exploiting Fluorescent Proteins to Understand Mycobacterium tuberculosis Biology             David Giacalone, Lu Huang, and Shumin Tan   18. Metabolic Labeling of Live Mycobacteria with Trehalose-Based Probes             Nicholas Banahene and Benjamin M. Swarts   19. Identification and Characterization of Mycobacterial Species Using Whole Genome Sequences             Marco A. Riojas, Andrew M. Frank, Samuel R. Greenfield, Stephen P. King, Conor J. Meehan, Michael Strong, Alice R. Wattam, and Manzour Hernando Hazbón   20. Whole Genome Sequencing of Mycobacterium tuberculosis Directly from Sputum Samples             Amanda Claire Brown   21. Experimental and Computational Workflow for RNA Sequencing in Mycobacterium tuberculosis: From Total RNA to Differentially Expressed Genes             Shuyi Ma, Richard M. Jones Jr., Natalie S. Gleason, Jessica Farrow-Johnson, and David R. Sherman   22. RNA Sequencing for Transcript 5′-End Mapping in Mycobacteria             M. Carla Martini, Huaming Sun, and Scarlet S. Shell   23. Methods for Proteomic Analyses of Mycobacteria             Carolina Mehaffy, Megan Lucas, Nicole A. Kruh-Garcia, and Karen M. Dobos   24. Targeted Lipidomics of Mycobacterial Lipids and Glycolipids             Emilie Layre   25. Metabolomics of Mycobacterium tuberculosis             Kyle A. Planck, Madhumitha Nandakumar, Luiz Pedro S. de Carvalho, and Kyu Rhee   26. Determining Minimum Inhibitory Concentrations in Liquid Cultures or on Solid Medium             Qinglan Wang and Helena I.M. Boshoff   27. A Multi-Stress Model for High Throughput Screening Against Non-Replicating Mycobacterium tuberculosis             Ben Gold, Thulasi Warrier, and Carl Nathan   28. Antimicrobial Susceptibility Testing for Mycobacterium sps in High Throughput Format             Esther Pérez-Herrán and Alfonso Mendoza   29. High-Content Analysis Monitoring Intracellular Trafficking and Replication of Mycobacterium tuberculosis Inside Host Cells             Nathalie Deboosere, Imène Belhaouane, Arnaud Machelart, Eik Hoffmann, Alexandre Vandeputte, and Priscille Brodin   30. Efficient Measurement of Drug Interactions with DiaMOND (Diagonal Measurement of N-Way Drug Interactions)             Nhi Van, Yonatan Degefu, and Bree B. Aldridge   31. Drug Sensitivity Testing of Mycobacterium tuberculosis Growing in a Hollow Fiber Bioreactor             Brett Mason and Véronique Dartois

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