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OverviewFluoroquinolones are one of the new classes of antimicrobial agents that are being increasingly used against tuberculosis, the most important ones are ciprofloxacin, ofloxacin, and the new generations drugs-gatifloxacin and moxifloxacin. The clinical isolates of Mycobacterium tuberculosis (47 ofloxacin- susceptible and 71 ofloxacin-resistant) strains obtained from individual patients from various parts of India were analyzed for gyr A mutation in quinolone resistant determining region (QRDR). Most of the mutations were seen clustered in the codons 90, 94 and 95, which is a hot spot region of QRDR. The types of mutations were correlated with the in vitro susceptibility pattern of the strains to ofloxacin. The resistance to fluoroquinolones was observed predominantly due to gyr A mutations. Gyr A being a gene with house keeping functions, is highly conserved in various mycobacterial species. It was adapted for PCR-RFLP assay using gyr A gene to see the difference in the profile of 23 standard species of mycobacteria. M.tuberculosis, M.avium, and M.intracellulare produced a different profile with two different restriction enzymes. Full Product DetailsAuthor: Sulochana Somasundaram , Paramasivan C N , Sujatha NPublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.40cm , Length: 22.90cm Weight: 0.118kg ISBN: 9783844397925ISBN 10: 3844397922 Pages: 72 Publication Date: 13 May 2011 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In stock We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |