|
|
|||
|
||||
OverviewA system for monitoring movement of the free-living second stage juveniles of root-knot nematodes (Meloidogyne spp.) using digital image analysis is described. The method is based on the analysis of video sequences of movement of individual nematodes encumbered with or without Pasteuria penetrans endospores. Software packages were used to grab video images, to process images and to monitor the movement of selected body part positions over time. The nematode centroid point and the nematode rectangular shape area were useful tools to estimate the locomotion. Data showed that a) the normal sinusoidal movement of nematodes is changed when individuals are encumbered with endospores of P. penetrans and b) in all cases a significant greater motility was observed by nematodes without P. penetrans endospores attached. Further, we modelled P. penetrans 'over-dispersion' in vitro and in vivo using the Poisson and the Negative Binomial distributions. The negative binomial distribution proved the most appropriate model to fit the observed data sets considering that P. penetrans spores are clumped. Full Product DetailsAuthor: Ioannis Vagelas , Barbara Pembroke , Simon R GowenPublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.60cm , Length: 22.90cm Weight: 0.163kg ISBN: 9783838377537ISBN 10: 3838377532 Pages: 104 Publication Date: 01 July 2010 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |